NDLI: Separation of somatropin charge variants by multiple-injection CZE with Polybrene/chondroitin sulfate A double-coated capillaries.

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Separation of somatropin charge variants by multiple-injection CZE with Polybrene/chondroitin sulfate A double-coated capillaries.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Amini, Ahmad
Description	Country affiliation: Sweden Author Affiliation: Amini A ( Medical Products Agency, and Division of Analytical Pharmaceutical Chemistry, Department of Medicinal Chemistry, Uppsala University, Biomedical Centre, Uppsala, Sweden. ahmad.amini@mpa.se)
Abstract	The performance of dynamic double-coated fused-silica capillaries with Polybrene and chondroitin sulfate A has been compared with uncoated fused-silica capillaries for the determination of recombinant human growth factor (somatropin) charge variants. The separations were carried out under the same electrophoretic conditions as described in the European Pharmacopoeia, i.e. at pH 6.0 and 30°C. The coating significantly reduced the interactions between the proteins and the surface of the fused-silica capillary. The first five separations performed in a new bare fused-silica capillary were discarded because of very poor separation performance as a result of protein-surface interactions. There was an approximate twofold increase in the interday migration time precision (%RSD ≤ 6.5%) in the double-coated capillaries. The method was successfully transferred to a multiple CZE mode where two samples were analyzed in a single electrophoretic run. The average purity of somatropin certified reference standard was 98.0% (%RSD ≤ 0.3%) determined by using uncoated and coated capillaries.
File Format	HTM / HTML
ISSN	16159306
Issue Number	16
Volume Number	36
e-ISSN	16159314
Journal	Journal of Separation Science
Language	English
Publisher	Wiley-VCH
Publisher Date	2013-08-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Analytical Discipline Chemistry Electrophoresis, Capillary Methods Human Growth Hormone Isolation & Purification Chondroitin Sulfates Chemistry Instrumentation Hexadimethrine Bromide Humans Evaluation Studies Journal Article
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Filtration and Separation

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