| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Zhang, Dan Park, Jin-A Kim, Dong-Soon Kim, Na-Hyun Kim, Seong-Kwan Cho, Kyeong-Su Jeong, Dana Shim, Jae-Han Abd El-Aty, A. M. Shin, Ho-Chul |
| Description |
Author Affiliation: Zhang D ( Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.); Park JA ( Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.); Kim DS ( Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.); Kim NH ( Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.); Kim SK ( Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.); Cho KS ( Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.); Jeong D ( Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.); Shim JH ( Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.); Abd El-Aty AM ( Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.); Shin HC ( Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.) |
| Abstract |
With the overarching aim to develop a simple and reliable method for the quantitative analysis of polypeptide antibiotics in various livestock products, the content of bacitracin, and polymyxin B in pork, beef, chicken, milk, and eggs was analyzed using colistin sulfate as an internal standard. The extracted samples were eluted via solid-phase extraction using 2% formic acid in acetonitrile/methanol (1:1, v/v). The two polypeptides were identified and quantified based on the intensities of mass fragments from the respective triply charged precursor ions (bacitracin: 474.97 amu and polymyxin B: 402 amu) at the defined retention time windows using liquid chromatography with electrospray ionization tandem mass spectrometry in time-scheduled multiple reaction monitoring mode. The calibration curves showed good linearity over the concentration range 50-2500 ng/mL with determination coefficients ≥ 0.991. The mean recoveries were in the range 80.3-88.8% with relative standard deviations <13% for all samples. The limits of quantitation ranged from 30-250 ng/g. The developed method was applied to market samples, but the target analytes were not detected in any of the samples. The developed method is reliable for the simultaneous detection of bacitracin and polymyxin B in pork, beef, chicken, milk, and eggs. |
| File Format |
HTM / HTML |
| ISSN |
16159306 |
| Issue Number |
14 |
| Volume Number |
38 |
| e-ISSN |
16159314 |
| Journal |
Journal of Separation Science |
| Language |
English |
| Publisher |
Wiley-VCH |
| Publisher Date |
2015-07-01 |
| Publisher Place |
Germany |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Analytical Discipline Chemistry Bacitracin Analysis Food Contamination Polymyxin B Animals Anti-bacterial Agents Cattle Chickens Chromatography, Liquid Eggs Food Analysis Livestock Milk Peptides Red Meat Reproducibility Of Results Solid Phase Extraction Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Analytical Chemistry Filtration and Separation |
