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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chen, Muwan Feng, Wenzhou Cao, Hui Zou, Lijin Chen, Chungui Baatrup, Anette Nielsen, Anne Bay Li, Haisheng Kassem, Moustapha Zou, Xuenong Bünger, Cody |
| Description | Country affiliation: Denmark Author Affiliation: Chen M ( Orthopaedic Research Lab, Aarhus University Hospital, 8000 Aarhus C, Denmark.) |
| Abstract | AIM OF THE STUDY: To investigate the effects of a traditional Chinese medicine (TCM) formula extract, named as ZD-I, on the proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro. MATERIALS AND METHODS: When hMSCs cultivated in the basal medium with ZD-I, cell viability was assessed by MTT assay and cellular proliferation was assessed by SYBR green I assay. The effects of ZD-I on osteogenic differentiation of hMSCs were assessed by alkaline phosphatase (ALP) activity, mineralization assay and real-time RT-PCR. RESULTS: ZD-I (0.78-100 microg/ml) was non-cytotoxic. The 50% inhibitory concentration (IC50) of hMSCs was 200 microg/ml. ZD-I (0.78-50 microg/ml) stimulated the proliferation of hMSCs. ZD-I did not change ALP activity of hMSCs cultivated in osteogenic medium in the early stage (4 and 7 days), but ZD-I inhibited the mineralization of hMSCs through down-regulation of several osteogenic markers (e.g. osteocalcin, bone morphogenetic protein 2 and osteopontin) in the late stage. CONCLUSIONS: ZD-I stimulate cellular proliferation and decrease the bone mineral deposition of hMSCs. These results suggest ZD-I may play an important therapeutic role in osteoarthritic patients by improving proliferative capacity of hMSCs and inhibiting the mineralization of hMSCs. |
| File Format | HTM / HTML |
| ISSN | 03788741 |
| Issue Number | 1 |
| Volume Number | 125 |
| e-ISSN | 18727573 |
| Journal | Journal of Ethnopharmacology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-08-17 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Ethnopharmacology Cell Differentiation Drug Effects Cell Proliferation Medicine, Chinese Traditional Mesenchymal Stromal Cells Osteoblasts Plant Extracts Pharmacology Alkaline Phosphatase Metabolism Culture Media Gene Expression Regulation Humans In Vitro Techniques Cytology Enzymology Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmacology |
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