| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Galdino, Pablinny M. Carvalho, Adryano A. V. Florentino, Iziara F. Martins, José L. R. Gazola, Andressa C. de Paula, José R. de Paula, Joelma A. M. Torres, Luce M. B. Costa, Elson A. de Lima, Thereza Christina M. |
| Spatial Coverage |
Brazil |
| Description |
Author Affiliation: Galdino PM ( Laboratório de Neurofarmacologia, Farmacologia, CCB, UFSC, Florianópolis, SC, Brazil); Carvalho AA ( Laboratório de Farmacologia de Produtos Naturais, ICB, UFG, Goiânia, GO, Brazil.); Florentino IF ( Laboratório de Farmacologia de Produtos Naturais, ICB, UFG, Goiânia, GO, Brazil.); Martins JL ( Laboratório de Farmacologia de Produtos Naturais, ICB, UFG, Goiânia, GO, Brazil.); Gazola AC ( Departamento de Ciências Farmacêuticas, CCS, UFSC, Florianópolis, SC, Brazil.); de Paula JR ( Laboratório de Pesquisa em Produtos Naturais, FF, UFG, Goiânia, GO, Brazil.); de Paula JA ( Unidade Universitária de Ciências Exatas e Tecnológicas, UEG, Anápolis, GO, Brazil.); Torres LM ( Instituto de Botânica, São Paulo, SP, Brazil.); Costa EA ( Laboratório de Farmacologia de Produtos Naturais, ICB, UFG, Goiânia, GO, Brazil.); de Lima TC ( Laboratório de Neurofarmacologia, Farmacologia, CCB, UFSC, Florianópolis, SC, Brazil.) |
| Abstract |
ETHNOPHARMACOLOGICAL RELEVANCE: Lafoensia pacari A. St.-Hil. (Lythraceae), known popularly as 'pacari' or 'mangaba-brava' is popularly used in the state of Goiás, Brazil. The stem bark or leaves are used to treat cancer, gastric disorders, inflammation and as a tonic to treat loss of enthusiasm. AIM OF THE STUDY: Previous results suggest that the ethanol:water 7:3 extract of the stem bark of L. pacari (PEx) has antidepressant-like activity in male mice. Our aim was to perform the PEx׳s bioguided fractionation and evaluate the monoaminergic system involvement in the antidepressant effect as well as progress in the study of L. pacari mechanism of action. MATERIAL AND METHODS: Mice (30-35g) orally treated (24, 5 and 1h) with PEx (100, 300 or 1000mg/kg), chloroform (ChloF-70mg/kg), ethyl acetate (180mg/kg), n-butanol (370mg/kg) and aqueous (1g/kg) fractions were submitted to the forced swimming test. To assess the mechanism of action, different groups of mice were pretreated with p-chlorophenylalanine (PCPA-100mg/kg, 4 days, i.p.) and alpha-methyl-p-tyrosine (AMPT-100mg/kg, 4h, i.p.) to assess the involvement of serotoninergic and catecholaminergic systems in the ChloF effects, respectively. A putative in vitro inhibition of monoamine oxidase (MAO) activity as well as the ex vivo hippocampal brain-derived neurotrophic factor (BDNF) quantification were carried out. Phytochemical screening, spectroscopy and chromatography analysis were used for identification of compounds present in ChloF. RESULTS AND DISCUSSION: After the fractionation, the ChloF 70mg/kg was the most active fraction, reducing the immobility time by 22%. Pre-treatments with both PCPA and AMPT abolished the ChloF effects, suggesting that ChloF antidepressant-like effect is dependent on serotonergic and catecholaminergic systems. ChloF did not inhibited MAO-A or MAO-B activity, excluding this as possible mechanism of action. ChloF augmented hippocampal BDNF level, which could be accounted for its antidepressant-like effect. Phytochemical screening showed the presence of saponins, tannins, steroids and triterpene in the PEx, and the presence of triterpene and steroids in ChloF. The spectroscopy and chromatography analysis identified lupeol, ß-sitosterol and stigmasterol in ChloF. CONCLUSION: ChloF is the fraction that better retained the crude extract active constituents. ChloF presents antidepressant-like effect that involves both serotonergic and catecholaminergic systems without inhibiting MAO enzymatic activity; this fraction also increases the hippocampal BDNF levels. |
| File Format |
HTM / HTML |
| ISSN |
03788741 |
| Volume Number |
170 |
| e-ISSN |
18727573 |
| Journal |
Journal of Ethnopharmacology |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-07-21 |
| Publisher Place |
Ireland |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Ethnopharmacology Antidepressive Agents Pharmacology Depression Drug Therapy Lythraceae Chemistry Plant Extracts Animals Administration & Dosage Isolation & Purification Brain-derived Neurotrophic Factor Metabolism Brazil Disease Models, Animal Dose-response Relationship, Drug Hippocampus Drug Effects Male Mice Monoamine Oxidase Serotonin Swimming Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Drug Discovery Pharmacology |
