| Author |
Pinto, Nícolas de Castro Campos Machado, Danielle Cunha da Silva, Josiane Mello Conegundes, Jéssica Leiras Mota Gualberto, Ana Cristina Moura Gameiro, Jacy Moreira Chedier, Luciana Castañon, Maria Christina Marques Nogueira Scio, Elita |
| Description |
Author Affiliation: Pinto Nde C ( Laboratory of Bioactive Natural Products, Department of Biochemistry, Biological Science Institute, Federal University of Juiz de Fora, 36036-900, Juiz de Fora, MG Brazil.); Machado DC ( Laboratory of Bioactive Natural Products, Department of Biochemistry, Biological Science Institute, Federal University of Juiz de Fora, 36036-900, Juiz de Fora, MG Brazil.); da Silva JM ( Laboratory of Bioactive Natural Products, Department of Biochemistry, Biological Science Institute, Federal University of Juiz de Fora, 36036-900, Juiz de Fora, MG Brazil.); Conegundes JL ( Laboratory of Bioactive Natural Products, Department of Biochemistry, Biological Science Institute, Federal University of Juiz de Fora, 36036-900, Juiz de Fora, MG Brazil.); Gualberto AC ( Department of Parasitology, Microbiology and Immunology, Biological Sciences Institute, Federal University of Juiz de Fora, 36036 900, Juiz de Fora, MG, Brazil.); Gameiro J ( Department of Parasitology, Microbiology and Immunology, Biological Sciences Institute, Federal University of Juiz de Fora, 36036 900, Juiz de Fora, MG, Brazil.); Moreira Chedier L ( Department of Botany, Biological Science Institute, Federal University of Juiz de Fora, 36036-900, Juiz de Fora, MG Brazil.); Castañon MC ( Department of Morphology, Biological Sciences Institute, Federal University of Juiz de Fora, 36036 900, Juiz de Fora, MG, Brazil.); Scio E ( Laboratory of Bioactive Natural Products, Department of Biochemistry, Biological Science Institute, Federal University of Juiz de Fora, 36036-900, Juiz de Fora, MG Brazil. Electronic address: elita.scio@ufjf.edu.br.) |
| Abstract |
ETHNOPHARMACOLOGICAL RELEVANCE: The leaves of Pereskia aculeata Miller (Cactaceae), known as Barbados gooseberry, are used in Brazilian traditional medicine as emollients and to treat skin wounds and inflammation. This study investigated the topical anti-inflammatory activity of the hexane fraction (HF) obtained from the methanol extract of the leaves of this species in models of acute and chronic ear dermatitis in mice. MATERIAL AND METHODS: Mice ear edema was induced by topical application of croton oil, arachidonic acid, capsaicin, ethyl-phenylpropiolate and phenol; and by subcutaneous injection of histamine. Ear biopsies were obtained to determine the levels of IL-1ß, IL-6 and TNF- cytokines by ELISA assay. Histopathological analysis was also performed to evaluate the HF activity in croton oil multiple application test. In addition, acute dermal irritation/corrosion test in rats was accomplished. HF chemical characterization was performed by GC-MS analysis. RESULTS: HF intensively reduced the inflammatory process induced by all irritant agents used, except for arachidonic acid. This activity is related, at least in part, to the reduction of IL-6 and TNF- cytokines levels. Moreover, when the glucocorticoid receptor antagonist mifepristone was used, HF failed to respond to the croton oil application.The results strongly suggested a glucocorticoid-like effect, which was reinforced by the presence of considerable amounts of sterol compounds identified in HF. The acute dermal irritaton/corrosion test showed no signs of toxicity. CONCLUSIONS: This study showed that the acute and chronic anti-inflammatory activity of P. aculeata leaves is very promising, and corroborates to better understand their ethnopharmacological applications. |
