NDLI: Flavone ingredients can synergistically inhibit NDV infecting cell and improve ND vaccine's protective rate.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yuqing, Zhang Zhan'ao, Sun Jiaguo, Liu Deyun, Wang Baokang, Zhang Fang, Yi Yunpeng, Fan Dan, Liu Xu, Liu Cui, Liu Yuanliang, Hu
Description	Author Affiliation: Yuqing Z ( Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. liujiaguo@njau.edu.cn)
Abstract	In order to screen better flavone prescriptions of anti-Newcastle disease virus (NDV), four flavone ingredients of epimedium flavones (EF), baikal skullcap root flavones (BSRF), wild dendranthema flower flavones (WDFF), and sanchi flavones (SF) screened in previous experiments and their prescriptions were added into chicken embryo fibroblast monolayer with three drug-adding modes respectively. The cellular A(570) values, the highest virus inhibitory rates and the score based on virus inhibitory rate were calculated to compare their antiviral activity. In immune protective test, the effects of three preparations (EF-BSRF, EF-SF-WDFF-BSRF and EF-WDFF-BSRF screened by the results in vitro experiment) on NDV infection were compared in chickens vaccinated with ND vaccine then challenged with NDV. Blood was regularly sampled for serum antibody titer determination. The pathogenic and dead statuses of chickens were clinically examined. The results indicated that the A(570) values of the nine prescriptions, especially the foresaid three prescriptions at almost all concentrations in three drug-adding modes were significantly higher than that of the virus control group. The foresaid three prescriptions presented at the top five of the highest virus inhibitory rate, and located at the highest three of the score rank. The antibody titers and protective rates of the three prescriptions groups were higher than that of VC group, especially EF-SF-WDFF-BSRF group showed significant difference. These results indicated that flavone prescriptions composed with suitable compatibility could possess synergistical action of antiviral effect, ES-SF-WDFF-BSRF prescription could inhibit the cellular infectivity of NDV, improve the protective effect of ND vaccine and would be expected to exploit into a new-type antiviral drug.
File Format	HTM / HTML
ISSN	01418130
Issue Number	3
Volume Number	51
e-ISSN	18790003
Journal	International Journal of Biological Macromolecules
Language	English
Publisher	Elsevier
Publisher Date	2012-10-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Antiviral Agents Pharmacology Flavones Newcastle Disease Prevention & Control Newcastle Disease Virus Drug Effects Immunology Viral Vaccines Animals Antibodies, Viral Blood Chick Embryo Chickens Virology Male Plant Extracts Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Structural Biology Molecular Biology Biochemistry

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