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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yamada, Masanori Kanamori, Yuka Yamada, Tetsuya |
| Description | Country affiliation: Japan Author Affiliation: Yamada M ( Department of Chemistry, Faculty of Science, Okayama University of Science, Ridaicho, Kita-Ku, Okayama 700-0005, Japan. myamada@chem.ous.ac.jp) |
| Abstract | We demonstrated the immobilization of double-stranded DNA onto the glass beads by psoralen, one of the DNA-intercalators in nature. As a result, DNA-immobilized glass beads (DNA-P-beads) were prepared by the intercalation of psoralen, which was immobilized onto the glass surface, onto the double-stranded DNA. These DNA-P-beads formed covalent bondings between psoralen and the nucleic acid base by 365 nm UV irradiation. The amount of immobilized-DNA was 0.24 mg per gram of glass beads. These DNA-P-beads were stable in water, and the DNA on the bead surface maintained its double-stranded structure. These DNA-P-beads selectively removed the planar-structure containing harmful compounds, such as dioxin and polychlorinated biphenyl (PCB) derivatives, from an aqueous multi-component solution. Additionally, a DNA-P-bead column effectively removed harmful compounds. Furthermore, the DNA-P-bead column could be reused by the addition of common organic solvents, such as ethanol. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Journal | International Journal of Biological Macromolecules |
| Volume Number | 60 |
| e-ISSN | 18790003 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-09-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Dna Chemistry Ficusin Glass Intercalating Agents Chemical Synthesis Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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