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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, Kun Li, Wei Rui, Xin Chen, Xiaohong Jiang, Mei Dong, Mingsheng |
| Description | Author Affiliation: Wang K ( College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.); Li W ( College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.); Rui X ( College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.); Chen X ( College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.); Jiang M ( College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.); Dong M ( College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: dongms@njau.edu.cn.) |
| Abstract | Two released exopolysaccharides (r-EPS1 and r-EPS2) were isolated from the culture supernatant of Lactobacillus plantarum 70810 by ethanol precipitation, anion exchange and gel filtration chromatography and characterization of the structures were conducted. The r-EPS1 and r-EPS2 were homogenous polysaccharides with average molecular weights of, respectively, 204.6 and 202.8kDa, and composed of glucose, mannose and galactose with molar ratios of 18.21:78.76:3.03 and 12.92:30.89:56.19, respectively. Methylation and NMR analysis revealed r-EPS1 was consisted of alpha configuration sugar residues, whereas r-EPS2 contained both alpha and beta configuration sugar residues. Antioxidant and antitumor activities of the two exopolysaccharides were evaluated in vitro. The results showed that r-EPS1 and r-EPS2 exhibited potent antioxidant activities regarding hydroxyl and DPPH radicals scavenging and reducing power assays, and possessed antitumor activities against Caco-2, BGC-823 and HT-29 cells, which suggested that they could be potential sources of antioxidant and antitumor drug. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 67 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Antioxidants Chemistry Lactobacillus Plantarum Polysaccharides Antineoplastic Agents Pharmacology Caco-2 Cells Ht29 Cells Humans Hydroxides Magnetic Resonance Spectroscopy Methylation Oxidation-reduction Isolation & Purification Metabolism Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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