NDLI: Interaction between intestinal dendritic cells and bacteria translocated from the gut in rats with $cirrhosis^{†}$

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Muñoz, Leticia José Borrero, María Ubeda, María Lario, Margaret Díaz, David Francés, Rubén Monserrat, Jorge Pastor, Oscar Aguado-Fraile, Elia Such, José Alvarez-Mon, Melchor Albillos, Agustín
Description	Country affiliation: Spain Author Affiliation: Muñoz L ( Departamento de Medicina, Universidad de Alcalá, Madrid, Spain.)
Abstract	Cirrhosis with ascites is associated with a high rate of gut bacterial translocation (GBT) and spontaneous bacterial infections of enteric origin. We addressed the activation state and role of intestinal dendritic cells (DCs) in experimental ascitic cirrhosis and their relationship with GBT. Cirrhosis with ascites was $CCl_{4}$ induced in rats. To examine their activation state and functions, DCs $(CD103^{+}RT1B^{+}CD3^{−}CD45RA^{−})$ were isolated from the intestinal lamina propria and mesenteric lymph nodes (MLNs), and the following parameters were determined by flow cytometry: surface antigen expression; spontaneous or lipopolysaccharide-stimulated tumor necrosis factor alpha (TNF-α) production; and in vitro capacity to phagocytose latex beads and to migrate toward the chemokine (C-C motif) ligand 21. GBT was defined as the growth of bacteria in MLNs culture. Bacterial DNA (Bact-DNA) in MLNs was identified by polymerase chain reaction. In rats with Bact-DNA in MLNs without GBT, intestinal and MLNs $CD103^{+}-DCs$ showed features of activation, expansion of the proinflammatory $CD4^{+}-DC$ subpopulation, augmented TNF-α production, and increased phagocytic and migratory capacities. In contrast, in rats with GBT, $CD103^{+}-DCs$ showed the absence of an activated phenotype, lowered TNF-α production, and relatively deficient phagocytosis and migration capacities. The $CD103^{+}-DC$ of rats without Bact-DNA in MLNs or GBT were similar to controls. In cirrhotic rats, bowel decontamination with antibiotics eliminated Bact-DNA in MLNs and GBT, normalized the activation state and functions of $CD103^{+}-DCs,$ and increased their TNF-α production. Conclusion: In experimental cirrhosis with ascites, continuous pressure of gut bacteria shapes the phenotypic and functional profile of intestinal DCs to produce effects that range from their activation and enhanced functions to their exhaustion and tolerance. (HEPATOLOGY 2012;56:1861–1869)
File Format	HTM / HTML
ISSN	02709139
Issue Number	5
Volume Number	56
e-ISSN	15273350
Journal	Hepatology
Language	English
Publisher	Wiley
Publisher Date	2012-11-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Hepatology Bacterial Translocation Immunology Dendritic Cells Metabolism Liver Cirrhosis Lymph Nodes Microbiology Animals Anti-bacterial Agents Pharmacology Antigens, Cd Antigens, Cd4 Ascites Chemically Induced Carbon Tetrachloride Cell Movement Cell Proliferation Dna, Bacterial Isolation & Purification Cytology Drug Effects Feces Integrin Alpha Chains Intestinal Mucosa Intestine, Small Male Mesentery Phagocytosis Rats Rats, Wistar Tumor Necrosis Factor-alpha Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Hepatology

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