NDLI: Zebrafish cdx1b regulates expression of downstream factors of Nodal signaling during early endoderm formation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cheng, Pei-Yi Lin, Chia-Chi Wu, Chun-Shiu Lu, Yu-Fen Lin, Che Yi Chung, Chih-Ching Chu, Cheng-Ying Huang, Chang-Jen Tsai, Chun-Yen Korzh, Svetlana Wu, Jen-Leih Hwang, Sheng-Ping L.
Description	Country affiliation: Taiwan Author Affiliation: Cheng PY ( Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan.)
Abstract	We identified a zebrafish caudal-related homeobox (cdx1b) gene, which shares syntenic conservation with both human and mouse Cdx1. Zebrafish cdx1b transcripts are maternally deposited. cdx1b is uniformly expressed in both epiblast and hypoblast cells from late gastrulation to the 1-2s stages and can be identified in the retinas, brain and somites during 18-22 hpf stages. After 28 hours of development, cdx1b is exclusively expressed in the developing intestine. Both antisense morpholino oligonucleotide-mediated knockdown and overexpression experiments were conducted to analyze cdx1b function. Hypoplastic development of the liver and pancreas and intestinal abnormalities were observed in 96 hpf cdx1b morphants. In 85% epiboly cdx1b morphants, twofold decreases in the respective numbers of gata5-, cas-, foxa2- and sox17-expressing endodermal precursors were identified. Furthermore, ectopic cdx1b expression caused substantial increases in the respective numbers of gata5-, cas-, foxa2- and sox17-expressing endodermal precursors and altered their distribution patterns in 85% epiboly injected embryos. Conserved Cdx1-binding motifs were identified in both gata5 and foxa2 genes by interspecific sequence comparisons. Cdx1b can bind to the Cdx1-binding motif located in intron 1 of the foxa2 gene based on an electrophoretic mobility shift assay. Co-injection of either zebrafish or mouse foxa2 mRNA with the cdx1b MO rescued the expression domains of ceruloplasmin in the liver of 53 hpf injected embryos. These results indicate that zebrafish cdx1b regulates foxa2 expression and may also modulate gata5 expression, thus affecting early endoderm formation. This study underscores a novel role of zebrafish cdx1b in the development of different digestive organs compared with its mammalian homologs.
File Format	HTM / HTML
ISSN	09501991
e-ISSN	14779129
Journal	Development
Issue Number	5
Volume Number	135
Language	English
Publisher	The Company of Biologists
Publisher Date	2008-03-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Developmental Discipline Biology Endoderm Physiology Gene Expression Regulation, Developmental Homeodomain Proteins Genetics Membrane Proteins Zebrafish Proteins Zebrafish Embryology Animals Genetic Vectors In Situ Hybridization Phylogeny Signal Transduction Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Developmental Biology Molecular Biology

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