NDLI: DEPS-1 promotes P-granule assembly and RNA interference in C. elegans germ cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Spike, Caroline A. Bader, Jason Reinke, Valerie Strome, Susan
Description	Country affiliation: United States Author Affiliation: Spike CA ( Department of Biology, Indiana University, Bloomington, IN 47405, USA.)
Abstract	P granules are germ-cell-specific cytoplasmic structures containing RNA and protein, and required for proper germ cell development in C. elegans. PGL-1 and GLH-1 were previously identified as critical components of P granules. We have identified a new P-granule-associated protein, DEPS-1, the loss of which disrupts P-granule structure and function. DEPS-1 is required for the proper localization of PGL-1 to P granules, the accumulation of glh-1 mRNA and protein, and germ cell proliferation and fertility at elevated temperatures. In addition, DEPS-1 is required for RNA interference (RNAi) of germline-expressed genes, possibly because DEPS-1 promotes the accumulation of RDE-4, a dsRNA-binding protein required for RNAi. A genome wide analysis of gene expression in deps-1 mutant germ lines identified additional targets of DEPS-1 regulation, many of which are also regulated by the RNAi factor RDE-3. Our studies suggest that DEPS-1 is a key component of the P-granule assembly pathway and that its roles include promoting accumulation of some mRNAs, such as glh-1 and rde-4, and reducing accumulation of other mRNAs, perhaps by collaborating with RDE-3 to generate endogenous short interfering RNAs (endo-siRNAs).
File Format	HTM / HTML
ISSN	09501991
e-ISSN	14779129
DOI	10.1242/dev.015552
Journal	Development
Issue Number	5
Volume Number	135
Language	English
Publisher	The Company of Biologists
Publisher Date	2008-03-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Developmental Discipline Biology Caenorhabditis Elegans Proteins Physiology Caenorhabditis Elegans Embryology Cytoplasmic Granules Embryo, Nonmammalian Rna, Messenger Genetics Animals Animals, Genetically Modified Cloning, Molecular Dna, Complementary Polymerase Chain Reaction Rna Interference Reverse Transcriptase Polymerase Chain Reaction Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Developmental Biology Molecular Biology

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