NDLI: Genome-wide view of TGFß/Foxh1 regulation of the early mesendoderm program.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chiu, William T. Charney Le, Rebekah Blitz, Ira L. Fish, Margaret B. Li, Yi Biesinger, Jacob Xie, Xiaohui Cho, Ken W. Y.
Description	Country affiliation: United States Author Affiliation: Chiu WT ( Department of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.); Charney Le R ( Department of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.); Blitz IL ( Department of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.); Fish MB ( Department of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.); Li Y ( Department of Computer Science, University of California, Irvine, CA 92697-2300, USA.); Biesinger J ( Department of Computer Science, University of California, Irvine, CA 92697-2300, USA.); Xie X ( Department of Computer Science, University of California, Irvine, CA 92697-2300, USA.); Cho KW ( Department of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA kwcho@uci.edu.)
Abstract	Nodal/TGFß signaling regulates diverse biological responses. By combining RNA-seq on Foxh1 and Nodal signaling loss-of-function embryos with ChIP-seq of Foxh1 and Smad2/3, we report a comprehensive genome-wide interaction between Foxh1 and Smad2/3 in mediating Nodal signaling during vertebrate mesendoderm development. This study significantly increases the total number of Nodal target genes regulated by Foxh1 and Smad2/3, and reinforces the notion that Foxh1-Smad2/3-mediated Nodal signaling directly coordinates the expression of a cohort of genes involved in the control of gene transcription, signaling pathway modulation and tissue morphogenesis during gastrulation. We also show that Foxh1 may function independently of Nodal signaling, in addition to its role as a transcription factor mediating Nodal signaling via Smad2/3. Finally, we propose an evolutionarily conserved interaction between Foxh1 and PouV, a mechanism observed in Pou5f1-mediated regulation of pluripotency in human embryonic stem and epiblast cells.
File Format	HTM / HTML
ISSN	09501991
e-ISSN	14779129
DOI	10.1242/dev.107227
Journal	Development
Issue Number	23
Volume Number	141
Language	English
Publisher	The Company of Biologists
Publisher Date	2014-12-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Developmental Discipline Biology Endoderm Embryology Forkhead Transcription Factors Metabolism Gene Expression Regulation, Developmental Physiology Mesoderm Transforming Growth Factor Beta Xenopus Proteins Xenopus Animals Blotting, Western Chromatin Immunoprecipitation Computational Biology Genetics Gene Knockdown Techniques Immunoprecipitation Morpholinos Nodal Protein Real-time Polymerase Chain Reaction Smad2 Protein Smad3 Protein Statistics, Nonparametric Research Support, N.i.h., Extramural Research Support, U.s. Gov't, P.h.s.
Content Type	Text
Resource Type	Article
Subject	Developmental Biology Molecular Biology

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