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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Vasina, Elena N. Déjardin, Philippe |
| Description | Country affiliation: France Author Affiliation: Vasina EN ( European Membrane Institute, UMR 5635 (CNRS, ENSCM, UM2), Université Montpellier 2, CC047, 2 Place Eugène Bataillon, F-34095 Montpellier Cedex 5, France. elena202@yahoo.com) |
| Abstract | Zeta potential and avidin adsorption of silicone elastomer (SE) and SE coated with polyethyleneimine (PEI) and heparin is studied under controlled hydrodynamic conditions. Zeta potential (ζ) of SE depends on the duration of contact with buffer (0.02M MOPS-KCl, pH 7.2): ζ descends from 12mV to a plateau value of -32mV during 5h and decreases further if SE is stretched (30% elongation). The time variation of ζ is attributed to surface charging of the core polymer PDMS. Coating the negatively charged SE with PEI results in ζ=42mV; a layer of heparin on top of PEI yields ζ=-46mV. On the negatively charged surfaces, avidin adsorption levels off at 5mV. Desorption of avidin from SE is negligible even when SE with adsorbed avidin is stretched, or when the stretching force is removed after adsorption of avidin. The results can be employed for designing biomaterials with properties such as surface charge and protein adsorption adjustable by stretching; the strong attachment of avidin can allow for further surface modification. |
| File Format | HTM / HTML |
| ISSN | 09277765 |
| Volume Number | 108 |
| e-ISSN | 18734367 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Chemistry Coated Materials, Biocompatible Chemistry Heparin Polyethyleneimine Silicone Elastomers Adsorption Avidin Hydrogen-ion Concentration Kinetics Microscopy, Atomic Force Static Electricity Surface Properties Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Colloid and Surface Chemistry Medicine Physical and Theoretical Chemistry Surfaces and Interfaces Biotechnology |
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