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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, Peipei Zhao, Xiaoliang Lv, Youjing Liu, Yanan Lang, Yinzhi Wu, Jiandong Liu, Xin Li, Miaomiao Yu, Guangli |
| Description | Country affiliation: China Author Affiliation: Wang P ( Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China); Zhao X ( Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China); Lv Y ( Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China); Liu Y ( Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China); Lang Y ( Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China); Wu J ( Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China); Liu X ( Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.); Li M ( Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.); Yu G ( Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China) |
| Abstract | A fucoidan from the brown alga Hizikia fusiforme was extracted with hot water and fractionated by anion-exchange chromatography. The main fraction YF5 was depolymerized by partial acid hydrolysis and further purified by gel filtration chromatograph. The sequences of oligosaccharides generated were then determined by ES-CID-MS/MS, which gave directly information on the structural heterogeneity of fucoidan from H. fusiforme. It was shown that YF5 had a backbone of a repeating disaccharide unit of [â4GlcAß1,2Man 1â], in good agreement with previous report by NMR and methylation analysis. In the product-ion spectra, the unique (0.2)A type fragmentation was important to establish the presence of a 2-linked Man in the backbone structure. In addition, abundant novel heterogeneous branched structural fragments were also detected and characterized: Fuc1,3Fuc, Fuc1,3Gal, Fuc1,4GlcA, Gal1,4Gal, Gal1,4GlcA and GlcA1,4GlcA. The sulfation mainly occurred at C2 or C4 of the fucose residue and C2, C4 or C6 of the galactose residue. |
| File Format | HTM / HTML |
| ISSN | 01448617 |
| Issue Number | 1 |
| Journal | Carbohydrate Polymers |
| Volume Number | 90 |
| e-ISSN | 18791344 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-09-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Materials Chemistry Polymers and Plastics |
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