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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nguyen, Anh Dzung Huang, Chun-Ching Liang, Tzu-Wen Nguyen, Van Bon Pan, Po-Shen Wang, San-Lang |
| Description | Author Affiliation: Nguyen AD ( Biotechnology Centre, Tay Nguyen University, 567 Le Duan Street, Buon Ma Thuot, Viet Nam.); Huang CC ( Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan.); Liang TW ( Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan); Nguyen VB ( Experimental Biology Department, Faculty of Natural Science and Technology, Tay Nguyen University, 567 Le Duan Street, Buon Ma Thuot, Viet Nam.); Pan PS ( Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan.); Wang SL ( Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan) |
| Abstract | Chitosanases have received much attention because of their wide range of applications. Although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (SPP)-containing Penicillium janthinellum D4 medium and purified by ammonium sulphate precipitation and combined column chromatography. The purified D4 chitosanase exhibited optimum activity at pH 7-9, 60°C and was stable at pH 7-11, 25-50°C. The D4 chitosanase that was used for chitooligomers preparation was studied. The enzyme products revealed various chitooligomers with different degrees of polymerisation (DP) from 3 to 9, as determined by a MALDI-TOF mass spectrometer, confirming the endo-type nature of the D4 chitosanase. D4 chitosanase activity was significantly inhibited by Cu(2+), Mn(2+), and EDTA. However, Fe(2+) activated or inhibited D4 chitosanases at different concentrations. The D4 chitosanase was also activated by some small synthetic boron-containing molecules with boronate ester side chains. |
| File Format | HTM / HTML |
| ISSN | 01448617 |
| Journal | Carbohydrate Polymers |
| Volume Number | 108 |
| e-ISSN | 18791344 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-08-08 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry__semicolon__materials Discipline Science Glycoside Hydrolases Metabolism Penicillium Chemistry Boron Chitin Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Materials Chemistry Polymers and Plastics |
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