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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Shankar, R. Madihah, M. S. Shaza, E. M. Nur Aswati, K. O. Suraini, A. A. Kamarulzaman, K. |
| Description | Country affiliation: Malaysia Author Affiliation: R S ( Industrial Biotechnology Department, Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.); M S M ( Industrial Biotechnology Department, Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. Electronic address: madihah@fbb.utm.my.); E M S ( Industrial Biotechnology Department, Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.); K O NA ( Industrial Biotechnology Department, Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.); A A S ( Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.); K K ( Industrial Biotechnology Research Centre, SIRIM Berhad, Malaysia.) |
| Abstract | The production of pullulanase by Bacillus flavothermus KWF-1 in batch and fed batch culture were compared using 2L bioreactor. In batch culture, 0.0803 U/mL of pullulanase activity with specific activity of 0.0213 U/mg was produced by controlling the agitation speed and temperature at 200 rpm and 50 °C, respectively. Fed batch production was studied by feeding the culture with different sago starch concentrations in various feeding modes for enhanced pullulanase production. Exponential feeding mode at dilution rate of 0.01/h was the preeminent strategy for enhanced pullulanase production of 0.1710 U/mL with specific activity of 0.066 U/mg. It had shown an increment of pullulanase production and specific activity by 2.1 and 3.1-fold, respectively when compared to batch culture. Increment of pullulanase activity in exponential feeding mode improved hydrolyzation of sago starch into maltotriose and panose by 4.5 and 2.5-fold respectively compared to batch system. |
| File Format | HTM / HTML |
| ISSN | 01448617 |
| Journal | Carbohydrate Polymers |
| Volume Number | 102 |
| e-ISSN | 18791344 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-02-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry__semicolon__materials Discipline Science Glycoside Hydrolases Biosynthesis Starch Metabolism Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Materials Chemistry Polymers and Plastics |
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