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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhao, Jingjin Zhang, Liangliang Chen, Chunfei Jiang, Jianhui Yu, Ruqin |
| Description | Author Affiliation: Zhao J ( State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, PR China.) |
| Abstract | Cancer is one of the most serious and lethal diseases around the world. Its early detection has become a challenging goal. To address this challenge, we developed a novel sensing platform using aptamer and RNA polymerase-based amplification for the detection of cancer cells. The assay uses the aptamer as a capture probe to recognize and bind the tumor marker on the surface of the cancer cells, forming an aptamer-based sandwich structure for collection of the cells in the microplate wells, and uses SYBR Green II dye as a tracer to produce strong fluorescence signal. The tumor marker interacts first with the recognition probes which were composed of the aptamer and single-stranded T7 RNA polymerase promoter. Then, the recognition probe hybridized with template probes to form a double-stranded T7 RNA polymerase promoter. This dsDNA region is extensively transcribed by T7 RNA polymerase to produce large amounts of RNAs, which are easily monitored using the SYBR Green II dye and a standard fluorometer, resulting in the amplification of the fluorescence signal. Using MCF-7 breast cancer cell as the model cell, the present sensing platform showed a linear range from 5.0×10(2) to 5.0×10(6) cells mL(-1) with a detection limit of 5.0×10(2) cells mL(-1). This work suggested a strategy to use RNA signal amplification combining aptamer recognition to develop a highly sensitive and selective method for cancer cells detection. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 745 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-10-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Aptamers, Nucleotide Chemistry Tumor Markers, Biological Analysis Biosensing Techniques Dna-directed Rna Polymerases Neoplasms Diagnosis Nucleic Acid Amplification Techniques Viral Proteins Animals Cho Cells Cricetinae Dna Metabolism Fluorescent Dyes Humans Limit Of Detection Mcf-7 Cells Organic Chemicals Promoter Regions, Genetic Rna Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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