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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chassy, Alexander W. Adams, Douglas O. Laurie, V. Felipe Waterhouse, Andrew L. |
| Description | Country affiliation: United States Author Affiliation: Chassy AW ( Department of Viticulture and Enology, One Shields Avenue, University of California, Davis, CA, USA.) |
| Abstract | Phenolic compounds in Vitis vinifera contribute important flavor, functionality, and health qualities to both table and wine grapes. The plant phenolic metabolic pathway has been well characterized, however many important questions remain regarding the influence of environmental conditions on pathway regulation. As a diagnostic for this pathway's regulation, we present a technique to incorporate a stable-isotopic tracer, L-phenyl-(13)C(6)-alanine (Phe(13)), into grape berries in situ and the accompanying high throughput analytical method based on LC-DAD-MS/MS to quantify and track the label into phenylalanine metabolites. Clusters of V. vinifera cv. Cabernet Sauvignon, either near the onset of ripening or 4 weeks later, were exposed to Phe(13) in the vineyard. Phe(13) was present in berries 9 days afterwards as well as labeled flavonols and anthocyanins, all of which possessed a molecular ion shift of 6 amu. However, nearly all the label was found in anthocyanins, indicating tight regulation of phenolic biosynthesis at this stage of maturity. This method provides a framework for examining the regulation of phenolic metabolism at different stages of maturity or under different environmental conditions. Additionally, this technique could serve as a tool to further probe the metabolism/catabolism of grape phenolics. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 747 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-10-17 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Anthocyanins Analysis Chromatography, High Pressure Liquid Methods Flavonols Phenols Tandem Mass Spectrometry Vitis Metabolism Carbon Isotopes Fruit Metabolic Networks And Pathways Physiology Phenylalanine Wine Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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