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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Najam-ul-Haq, Muhammad Jabeen, Fahmida Hussain, Dilshad Saeed, Adeela Musharraf, Syed Ghulam Huck, Christian W. Bonn, Günther K. |
| Description | Country affiliation: Pakistan Author Affiliation: Najam-ul-Haq M ( Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan. najamulhaq@bzu.edu.pk) |
| Abstract | Protein phosphorylation is one of the most important post-translational modifications. Phosphorylated peptides are present in low abundance in blood serum but play a vital role in regulatory mechanisms and may serve as casual factors in diseases. The enrichment and analysis of phosphorylated peptides directly from human serum and mapping the phosphorylation sites is a challenging task. Versatile nanocomposites of different materials have been synthesized using simple but efficient methodologies for their enrichment. The nanocomposites include magnetic, coated, embedded as well as chemically derivatized materials. Different base materials such as polymers, carbon based and metal oxides are used. The comparison of nanocomposites with respective nanoparticles provides sufficient facts about their efficiency in terms of loading capacity and capture efficiency. The cost for preparing them is low and they hold great promise to be used as chromatographic materials for phosphopeptide enrichment. This review gives an overview of different nanocomposites in phosphoproteomics, discussing the improved efficiency than the individual counterparts and highlighting their significance in phosphopeptide enrichment. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 747 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-10-17 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Biological Markers Blood Chromatography, Affinity Instrumentation Nanocomposites Chemistry Phosphopeptides Isolation & Purification Aluminum Oxide Methods Humans Magnetite Nanoparticles Microspheres Phosphorylation Protein Processing, Post-translational Proteomics Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Titanium Journal Article Research Support, Non-u.s. Gov't Review |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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