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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Raj, M. Amal John, S. Abraham |
| Description | Country affiliation: India Author Affiliation: Raj MA ( Centre for Nanoscience and Nanotechnology, Department of Chemistry, Gandhigram Rural Institute, Gandhigram, Dindigul, Tamilnadu, India.) |
| Abstract | This paper describes the fabrication of graphene on glassy carbon electrode (GCE) by electrochemical reduction of graphene oxide (GO) attached through 1,6-hexadiamine on GCE and the simultaneous determination of structurally similar four purine derivatives using the resultant electrochemically reduced GO (ERGO) modified electrode. The electrocatalytic activity of ERGO was investigated toward the oxidation of four important purine derivatives, uric acid (UA), xanthine (XN), hypoxanthine (HXN) and caffeine (CAF) at physiological pH. The modified electrode not only enhanced the oxidation currents of the four purine derivatives but also shifted their oxidation potentials toward less positive potentials in contrast to bare GCE. Further, it successfully separates the voltammetric signals of the four purine derivatives in a mixture and hence used for the simultaneous determination of them. Selective determination of one purine derivative in the presence of low concentrations other three purine derivatives was also realized at the present modified electrode. Using differential pulse voltammetry, detection limits of 8.8×10(-8)M, 1.1×10(-7)M, 3.2×10(-7)M and 4.3×10(-7)M were obtained for UA, XN, HXN and CAF, respectively. The practical application of the modified electrode was demonstrated by simultaneously determining the concentrations of UA, XN, HXN and CAF in human blood plasma and urine samples. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 771 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-04-10 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Blood Chemical Analysis Methods Graphite Chemistry Oxides Purines Blood Urine Urinalysis Instrumentation Caffeine Catalysis Electrochemistry Electrodes Humans Hypoxanthine Oxidation-reduction Spectrum Analysis, Raman Time Factors Uric Acid Xanthine Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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