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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Longuespée, Rémi Wefers, Annika K. De Vita, Elena Miller, Aubry K. Reuss, David E. Wick, Wolfgang Herold-Mende, Christel Kriegsmann, Mark Schirmacher, Peter von Deimling, Andreas Pusch, Stefan |
| Abstract | All isocitrate dehydrogenase (IDH) mutant solid neoplasms exhibit highly elevated levels of D-2-hydroxyglutarate (D-2HG). Detection of 2HG in tumor tissues currently is performed by gas or liquid chromatography-mass spectrometry (GC- or LC-MS) or biochemical detection. While these methods are highly accurate, a considerable amount of time for tissue preparation and a relatively high amount of tissue is required for testing. We here present a rapid approach to detect 2HG in brain tumor tissue based on matrix-assisted laser desorption ionization - time of flight mass spectrometry (MALDI-TOF). We analyzed 26 brain tumor samples with known IDH1 or IDH2 mutation and compared readouts to those from 28 brain tumor samples of wildtype IDH status. IDH mutant samples exhibited a clear positive signal for 2HG which was not observed in any of the IDH wildtype tumors. Our analytical pipeline allowed for 2HG detection in less than 5 min. Data were validated by determining 2HG levels in all tissues with a biochemical assay. In conclusion, we developed a protocol for rapid detection of 2HG levels and illustrate the possibility to use MALDI-TOF for the detection of metabolites on frozen tissue sections in a diagnostic setting. |
| Related Links | https://actaneurocomms.biomedcentral.com/counter/pdf/10.1186/s40478-018-0523-3.pdf |
| Ending Page | 9 |
| Page Count | 9 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 20515960 |
| DOI | 10.1186/s40478-018-0523-3 |
| Journal | Acta Neuropathologica Communications |
| Issue Number | 1 |
| Volume Number | 6 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2018-03-02 |
| Access Restriction | Open |
| Subject Keyword | Neurosciences Pathology Neurology IDH mutation 2-hydroxyglutarate Diffuse glioma MALDI-TOF Mass spectrometry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pathology and Forensic Medicine Neurology (clinical) Cellular and Molecular Neuroscience |
| Journal Impact Factor | 6.2/2023 |
| 5-Year Journal Impact Factor | 6.8/2023 |
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