NDLI: Cefquinome shows a higher impact on the pig gut microbiome and resistome compared to ceftiofur

Content Provider	Springer Nature : BioMed Central
Author	Rutjens, Sofie Vereecke, Nick Sauer, Jannes Croubels, Siska Devreese, Mathias
Abstract	Cephalosporins are licensed for treatment of severe bacterial infections in different species. However, the effect of these antimicrobials on the fecal microbiome and potential spread of resistance-associated genes causes great concern. This highlights the need to understand the impact of cephalosporins on the porcine fecal microbiome and resistome. A combination of long-read 16S rRNA gene and shotgun metagenomic sequencing was applied to investigate the effect of conventional treatment with either ceftiofur (3 mg.kg−1 intramuscular, 3 consecutive days) or cefquinome (2 mg.kg−1 intramuscular, 5 consecutive days) on the porcine microbiome and resistome. Fecal samples were collected from 17 pigs (6 ceftiofur treated, 6 cefquinome treated, 5 control pigs) at four different timepoints. Treatment with ceftiofur resulted in an increase in Proteobacteria members on microbiome level, while on resistome level selection in TetQ containing Bacteroides, CfxA6 containing Prevotella and blaTEM-1 containing Escherichia coli was observed. Cefquinome treatment resulted in a decline in overall species richness (α-diversity) and increase in Proteobacteria members. On genus level, administration of cefquinome significantly affected more genera than ceftiofur (18 vs 8). On resistome level, cefquinome resulted in a significant increase of six antimicrobial resistance genes, with no clear correlation with certain genera. For both antimicrobials, the resistome levels returned back to the control levels 21 days post-treatment. Overall, our study provides novel insights on the effect of specific cephalosporins on the porcine gut microbiome and resistome after conventional intramuscular treatment. These results might contribute to better tailoring of the most ideal treatment strategy for some bacterial infections.
Related Links	https://veterinaryresearch.biomedcentral.com/counter/pdf/10.1186/s13567-023-01176-8.pdf
Ending Page	17
Page Count	17
Starting Page	1
File Format	HTM / HTML
ISSN	12979716
DOI	10.1186/s13567-023-01176-8
Journal	Veterinary Research
Issue Number	1
Volume Number	54
Language	English
Publisher	BioMed Central
Publisher Date	2023-06-06
Access Restriction	Open
Subject Keyword	Veterinary Medicine Veterinary Science Virology Microbiology Immunology Veterinary cephalosporins gut microbiome resistome ARGs porcine feces 16S rRNA sequencing shotgun sequencing metagenomics Veterinary Medicine/Veterinary Science
Content Type	Text
Resource Type	Article
Subject	Veterinary Medicine
Journal Impact Factor	3.7/2023
5-Year Journal Impact Factor	3.8/2023

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