NDLI: Production, statistical evaluation and characterization of chitosanase from Fusarium oxysporum D18

Content Provider	Springer Nature : BioMed Central
Author	Abedin, Rania M. A. Abd Elwaly, Doha R. M. Abd El-Salam, Ayat E.
Abstract	Purpose The present research work focuses on the extraction of chitosanase enzyme from soil fungi. Chitosan hydrolysis by chitosanase is one of the most effective methods to produce chitosan oligosaccharides which are new biomaterials that have many biological activities such as antitumour, antioxidant, antidiabetic and antimicrobial. Method A strain producing chitosanase was screened and identified as Fusarium oxysporum D18 with an accession number OL343607. Various physiological parameters (incubation type, carbon source, additive nitrogen source, statistical evaluation, solid state fermentation) were assessed to increase chitosanase production. Results Fusarium oxysporum D18 produced a considerable value of chitosanase (1.220 U/ml). After 7 days of incubation, the best carbon source was lactose, and the best nitrogen source was ammonium chloride. Statistical evaluation was carried out by using Plackett–Burman and Box-Behnken designs. The highest chitosanase production (1.994 U/ml) was induced by the medium composition g/l: KH2PO4 (1.5), MgSO4 (0.269), lactose (18), NH4Cl (1.26), pH (6.68), using a 5-day-old inoculum and chitosanase activity was 1.63 folds that of the original medium. The production of chitosanase by Fusarium oxysporum D18 in solid state cultures using different solid substrates was studied and the best solid substrate for higher chitosanase activity (2.246 U/ml) was raw shrimp heads and shells and chitosanase activity was 1.13 folds that of the optimized liquid cultures. An extracellular chitosanase was isolated and partially purified by using 75% saturation of ammonium sulphate. The highest chitosanase activity (3.667 U/ml) with a specific activity of 0.390 U/mg protein was obtained at enzyme protein concentration of 9.391 mg/ml, substrate concentration of 1.2 % (w/v), Vmax of the enzyme of approximately 0.430 U/mg protein, and KM of 0.26 % (w/v), at pH 5.6 and reaction temperature of 50 °C. The activity of the purified and characterized chitosanase increased by 3 times than that the original isolate activity. The enzyme was thermostable and retained about 55% of its original activity after heating at 70 °C for 15 min. The enzyme preparations were activated by Ca2+ ions and inactivated by Zn+2, Cu+2 ions, and EDTA. Conclusion An antitumour activity of chitooligosaccharides produced by the chitosanase was applied to the MCF-7 (breast carcinoma cells) and they had a cytotoxicity inhibitory effect against them about IC50 = 448 μg/ml.
Related Links	https://annalsmicrobiology.biomedcentral.com/counter/pdf/10.1186/s13213-023-01731-w.pdf
Ending Page	14
Page Count	14
Starting Page	1
File Format	HTM / HTML
ISSN	18692044
DOI	10.1186/s13213-023-01731-w
Journal	Annals of Microbiology
Issue Number	1
Volume Number	73
Language	English
Publisher	BioMed Central
Publisher Date	2023-08-19
Access Restriction	Open
Subject Keyword	Microbiology Microbial Genetics and Genomics Microbial Ecology Mycology Applied Microbiology Chitosanase Chitosan Chitooligosaccharides (COS) Solid state fermentation (SSF) Anticancer activity
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology
Journal Impact Factor	3/2023
5-Year Journal Impact Factor	3.4/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Isolation of a thermophilic Anoxybacillus flavithermus sp. nov. and production of thermostable α-amylase under solid-state fermentation (SSF)

Statistical optimization of cellulases production by Aspergillus niger HQ-1 in solid-state fermentation and partial enzymatic characterization of cellulases on hydrolyzing chitosan

Statistical optimization of chitosanase production by Aspergillus sp. QD-2 in submerged fermentation

Erratum to: Cellulase production by solid state fermentation using bagasse with Penicillium decumbens L-06

Production of pectinolytic enzymes pectinase and pectin lyase by Bacillus subtilis SAV-21 in solid state fermentation

Effect of different cultivation conditions and inducers on the production of laccase by the litter-dwelling fungal isolate Fusarium incarnatum LD-3 under solid substrate fermentation

Solid-state fermentation with Serratia marcescens Xd-1 enhanced production of prodigiosin by using bagasse as an inertia matrix

Exoglucanase production by Aspergillus niger grown on wheat bran : Exoglucanase by A. niger in SSF

Inulinase production by Saccharomyces sp. in solid state fermentation using wheat bran as substrate

Production, statistical evaluation and characterization of chitosanase from Fusarium oxysporum D18

Similar Documents

Isolation of a thermophilic Anoxybacillus flavithermus sp. nov. and production of thermostable α-amylase under solid-state fermentation (SSF)

Statistical optimization of cellulases production by Aspergillus niger HQ-1 in solid-state fermentation and partial enzymatic characterization of cellulases on hydrolyzing chitosan

Statistical optimization of chitosanase production by Aspergillus sp. QD-2 in submerged fermentation

Erratum to: Cellulase production by solid state fermentation using bagasse with Penicillium decumbens L-06

Production of pectinolytic enzymes pectinase and pectin lyase by Bacillus subtilis SAV-21 in solid state fermentation

Effect of different cultivation conditions and inducers on the production of laccase by the litter-dwelling fungal isolate Fusarium incarnatum LD-3 under solid substrate fermentation

Solid-state fermentation with Serratia marcescens Xd-1 enhanced production of prodigiosin by using bagasse as an inertia matrix

Exoglucanase production by Aspergillus niger grown on wheat bran : Exoglucanase by A. niger in SSF

Inulinase production by Saccharomyces sp. in solid state fermentation using wheat bran as substrate

Production, statistical evaluation and characterization of chitosanase from Fusarium oxysporum D18