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Production of pectinolytic enzymes pectinase and pectin lyase by Bacillus subtilis SAV-21 in solid state fermentation

Content Provider	Springer Nature Link
Author	Kaur, Simran Jot Gupta, Vijay Kumar
Copyright Year	2017
Abstract	Pectin-degrading enzymes (pectinase and pectin lyase) were produced in solid state fermentation by Bacillus subtilis SAV-21 isolated from fruit and vegetable market waste soil of Yamuna Nagar, Haryana, India, and identified by 16S rDNA sequencing. Under optimized conditions, maximum production of pectinase (3315 U/gds) and pectin lyase (10.5 U/gds) was recorded in the presence of a combination of orange peel and coconut fiber (4:1), with a moisture content of 60% at 35 °C and pH 4.0 after 4 days and 8 days of incubation, respectively. Pectinase yield was enhanced upon supplementation with galactose and yeast extract, whereas pectin lyase production was unaffected by adding carbon and nitrogen source to the basal medium. Thus, B. subtilis SAV-21 can be exploited for cost-effective production of pectinase and pectin lyase using agro-residues.
Starting Page	333
Ending Page	342
Page Count	10
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	67
Issue Number	4
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2017-03-28
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Coconut fiber Pectinase Pectin lyase Solid state fermentation Optimization Microbiology Microbial Genetics and Genomics Microbial Ecology Mycology Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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