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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Ramirez-Lopez, Camilo Jose Barros, Edvaldo Vidigal, Pedro Marcus Gomes, Lidiany Lopes Baracat-Pereira, Maria Cristina Guimarães, Simone Eliza Facioni Guimarães, José Domingos |
| Abstract | Objectives Bovine seminal plasma proteins perform several functions related to sperm function. Changes in the expression pattern or abundance of seminal proteins are related to changes in the fertilizing capacity of bulls. Considering the role of seminal plasma proteins in sperm function and animal reproduction, we investigated changes in the protein abundance profile in response to sperm morphological changes using a proteomic approach. Datadescription In our present investigation, we employed liquid chromatography coupled with mass spectrometry to elucidate the proteomic composition of seminal plasma obtained from Nellore bulls exhibiting varying percentages of sperm abnormalities. Following semen collection, seminal plasma was promptly isolated from sperm, and proteins were subsequently precipitated, enzymatically digested using porcine trypsin, and subjected to analysis utilizing the Acquity nano UHPLC System in conjunction with a mass spectrometer. This dataset encompasses a total of 297 proteins, marking the inaugural instance in which a comparative profile of seminal plasma proteins in young Nellore bulls, categorized by their sperm abnormality percentages, has been delineated using LC-MS/MS. The comprehensive nature of this dataset contributes pivotal proteomic insights, representing a noteworthy advancement in our understanding of the reproductive biology of the Nellore breed. |
| Related Links | https://bmcresnotes.biomedcentral.com/counter/pdf/10.1186/s13104-024-06796-7.pdf |
| Ending Page | 4 |
| Page Count | 4 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 17560500 |
| DOI | 10.1186/s13104-024-06796-7 |
| Journal | BMC Research Notes |
| Issue Number | 1 |
| Volume Number | 17 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2024-05-10 |
| Access Restriction | Open |
| Subject Keyword | Biomedicine Medicine Public Health Life Sciences Proteomic Mass spectrometry Bos indicus Bovine Sire Semen Medicine/Public Health |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology Medicine |
| Journal Impact Factor | 1.6/2023 |
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