NDLI: and Kedah Kelantan (Bos indicus) Sperm Proteome Identifies Sperm Proteins Potentially Responsible for Higher Fertility in a Tropical Climate (2013)

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and Kedah Kelantan (Bos indicus) Sperm Proteome Identifies Sperm Proteins Potentially Responsible for Higher Fertility in a Tropical Climate (2013)

Content Provider	CiteSeerX
Author	Karsani, Saiful Anuar Ashrafzadeh, Ali Nathan, Sheila
Abstract	Abstract: The fertility of zebu cattle (Bos indicus) is higher than that of the European purebred (Bos taurus) and crossbred (Bos taurus × Bos indicus) cattle in tropical areas. To identify proteins related to the higher thermo-tolerance and fertility of Zebu cattle, this study was undertaken to identify differences in sperm proteome between the high fertile Malaysian indigenous zebu cattle (Kedah Kelantan) and the sub-fertile crossbred cattle (Mafriwal). Frozen semen from three high performance bulls from each breed were processed to obtain live and pure sperm. Sperm proteins were then extracted, and two-dimensional gel electrophoresis performed to compare proteome profiles. Gel image analysis identified protein spots of interest which were then identified by liquid chromatography mass spectrometry quadrupole time-of-flight (LC MS/MS Q-TOF). STRING network analysis predicted interactions between at least 20 of the identified proteins. Among the identified proteins, a number of motility and energy related proteins were present in greater abundance in Kedah Kelantan. Sperm motility evaluation by Computer Assisted Semen Analysis (CASA) confirmed significantly higher motility in Kedah Kelantan. While results from this study do identify proteins that may be responsibleInt. J. Mol. Sci. 2013, 14 15861
File Format	PDF
Publisher Date	2013-01-01
Access Restriction	Open
Subject Keyword	Frozen Semen Protein Spot High Performance Bull Bos Taurus Bos Indicus Energy Related Protein Gel Image Analysis Bos Taurus Pure Sperm Two-dimensional Gel Electrophoresis Kedah Kelantan Higher Fertility Tropical Climate Sperm Motility Evaluation Proteome Profile Sperm Proteome Computer Assisted Semen Analysis Sub-fertile Crossbred Cattle Zebu Cattle Identified Protein Bos Indicus Lc M M Q-tof Tropical Area Sperm Protein String Network Analysis
Content Type	Text
Resource Type	Article

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