NDLI: Verification of dried blood spot as a sample type for HIV viral load and early infant diagnosis on Hologic Panther in Zambia

Content Provider	Springer Nature : BioMed Central
Author	Simushi, Precious Kalunga, Mukoshya Nchima Mwakyoma, Tuku Mwewa, Mulenga Muchaili, Lweendo Hazeemba, Nchimunya Mulenga, Chileshe Mwewa, Patience Chiyenu, Kaseya O. R. Kachimba, John Choonga, Powell Shibemba, Aaron Hamooya, Benison M. Zambwe, Mowa Chipimo, Peter J. Kasonka, Lackson
Abstract	Objective Zambia has embarked on improving the diagnostic capacity by setting up high throughput and accurate machines in the testing process and introduction of dried blood spot (DBS) as a sample type. This was a cross sectional study to verify dried blood spot as a sample type for HIV viral load and early infant diagnosis (EID) on Hologic Panther platform and Evaluate the analytical performance (precision, linearity and measurement of uncertainty) of the Hologic Panther. Results The specificity and sensitivity of EID performance of Aptima Quant Dx assay on Hologic panther machine against the gold standard machine COBAS Taqman (CAP/CTM) was 100% with an overall agreement of 100%. The quantitative HIV Viral Load (VL) accuracy had a positive correlation of (0.96) obtained against the gold standard (plasma samples) run on COBAS4800 platform. Analytical performance of the Hologic panther machine was evaluated; Precision low positive repeatability 3.50154 and within lab 2.268915 at mean 2.88 concentration and precision high positive repeatability 1.116955 and within lab 2.010677 at mean 5.09 concentration were obtained confirming manufacturers claims. Uncertainty of measurement for this study was found to be ± 71 copies/ml. Linearity studies were determined and all points were within acceptable limits. We therefore recommend DBS as a sample type alternative to plasma for the estimation of HIV-1 viral load and EID diagnosis on the Hologic panther machine.
Related Links	https://bmcresnotes.biomedcentral.com/counter/pdf/10.1186/s13104-023-06344-9.pdf
Ending Page	6
Page Count	6
Starting Page	1
File Format	HTM / HTML
ISSN	17560500
DOI	10.1186/s13104-023-06344-9
Journal	BMC Research Notes
Issue Number	1
Volume Number	16
Language	English
Publisher	BioMed Central
Publisher Date	2023-05-11
Access Restriction	Open
Subject Keyword	Biomedicine Medicine Public Health Life Sciences Dried blood spot Early infant diagnosis HIV viral load Hologic Panther Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology Medicine
Journal Impact Factor	1.6/2023

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