NDLI: Relationship of Leishmania RNA Virus (LRV) and treatment failure in clinical isolates of Leishmania major

Content Provider	Springer Nature : BioMed Central
Author	Abtahi, Mohsen Eslami, Gilda Cavallero, Serena Vakili, Mahmood Hosseini, Saeedeh Sadat Ahmadian, Salman Boozhmehrani, Mohammad Javad Khamesipour, Ali
Abstract	Objective Leishmaniasis is caused by different Leishmania spp. Treatment failure (TF) of cutaneous leishmaniasis (CL) is a serious issue that may be due to various reasons, previous studies suggested Leishmania RNA virus (LRV) as a potential cause of TF. Two variant groups of LRV1 and LRV2 are reported. In this study, the presence of LRV1/LRV2 was compared in TF with treatment response (TR) isolates of L. major. Clinical isolates of 15 TF and 15 TR were collected from CL patients referred to the Health Centers of Isfahan. Genomic DNA was extracted to identify Leishmania spp. using ITS1-PCR–RFLP. Identification of LRV1/LRV2 was performed using SYBR Green Real-Time PCR. The statistical analysis to test relationship between the treatment response with Glucantime and the presence of LRV were performed using SPSS 16.0 with Fisher’s Exact test. P value of less than 0.05 was considered significant. Results ITS1-PCR–RFLP results showed that every isolate was identified as L. major. The results showed no LRV1 in any of the samples but 7 TR isolates and 2 TF isolates showed positive for LRV2. Statistical analysis showed no significant difference between the presence of LRV2 and response to Glucantime (p-value = 0.1086). Therefore, other mechanisms might be responsible for TF.
Related Links	https://bmcresnotes.biomedcentral.com/counter/pdf/10.1186/s13104-020-04973-y.pdf
Ending Page	6
Page Count	6
Starting Page	1
File Format	HTM / HTML
ISSN	17560500
DOI	10.1186/s13104-020-04973-y
Journal	BMC Research Notes
Issue Number	1
Volume Number	13
Language	English
Publisher	BioMed Central
Publisher Date	2020-03-04
Access Restriction	Open
Subject Keyword	Biomedicine Medicine Public Health Life Sciences Leishmania major Cutaneous Leishmaniasis Leishmania RNA Virus Treatment Failure SYBR Green Real-Time PCR Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology Medicine
Journal Impact Factor	1.6/2023

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