NDLI: Evaluation of two Plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates

Content Provider	Springer Nature : BioMed Central
Author	Zhang, Yongzhe Liu, Fei Zhao, Yan Yang, Fan Bai, Jie Jia, Xitong Roobsoong, Wanlapa Sattabongkot, Jetsumon Cui, Liwang Cao, Yaming Luo, Enjie Wang, Meilian
Abstract	Background Plasmodium vivax transmission-blocking vaccines (TBVs) are receiving increasing attention. Based on excellent transmission-blocking activities of the PbPH (PBANKA_0417200) and PbSOP26 (PBANKA_1457700) antigens in Plasmodium berghei, their orthologs in P. vivax, PVX_098655 (PvPH) and PVX_101120 (PvSOP26), were selected for the evaluation of their potential as TBVs. Methods Fragments of PvPH (amino acids 22–304) and PvSOP26 (amino acids 30–272) were expressed in the yeast expression system. The recombinant proteins were used to immunize mice to obtain antisera. The transmission-reducing activities of these antisera were evaluated using the direct membrane feeding assay (DMFA) using Anopheles dirus mosquitoes and P. vivax clinical isolates. Results The recombinant proteins PvPH and PvSOP26 induced robust antibody responses in mice. The DMFA showed that the anti-PvSOP26 sera significantly reduced oocyst densities by 92.0 and 84.1% in two parasite isolates, respectively, whereas the anti-PvPH sera did not show evident transmission-reducing activity. The variation in the DMFA results was unlikely due to the genetic polymorphisms of the two genes since their respective sequences were identical in the clinical P. vivax isolates. Conclusion PvSOP26 could be a promising TBV candidate for P. vivax, which warrants further evaluation. Graphical Abstract
Related Links	https://parasitesandvectors.biomedcentral.com/counter/pdf/10.1186/s13071-021-04909-w.pdf
Ending Page	9
Page Count	9
Starting Page	1
File Format	HTM / HTML
ISSN	17563305
DOI	10.1186/s13071-021-04909-w
Journal	Parasites & Vectors
Issue Number	1
Volume Number	14
Language	English
Publisher	BioMed Central
Publisher Date	2021-08-16
Access Restriction	Open
Subject Keyword	Parasitology Entomology Tropical Medicine Infectious Diseases Veterinary Medicine Veterinary Science Virology Plasmodium vivax Transmission-blocking vaccine Yeast expression Direct membrane feeding assay Veterinary Medicine/Veterinary Science
Content Type	Text
Resource Type	Article
Subject	Veterinary Infectious Diseases Parasitology
Journal Impact Factor	3/2023
5-Year Journal Impact Factor	3.3/2023

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