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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Li, Jizhen Zhao, Lingling Wu, Zerui Huang, Shirui Wang, Junyu Chang, Yuanyuan Liu, Li Jin, Honglei Lu, Jianglong Huang, Chuanshu Xie, Qipeng Huang, Haishan Su, Zhipeng |
| Abstract | Background Glioblastoma (GB) is recognized as one of the most aggressive brain tumors, with a median survival of 14.6 months. However, there are still some patients whose survival time was greater than 3 years, and the biological reasons behind this clinical phenomenon arouse our research interests. By conducting proteomic analysis on tumor tissues obtained from GB patients who survived over 3 years compared to those who survived less than 1 year, we identified a significant upregulation of SelK in patients with shorter survival times. Therefore, we hypothesized that SelK may be an important indicator related to the occurrence and progression of GBM. Methods Proteomics and immunohistochemistry from GB patients were analyzed to investigate the correlation between SelK and clinical prognosis. Cellular phenotypes were evaluated by cell cycle analysis, cell viability assays, and xenograft models. Immunoblots and co-immunoprecipitation were conducted to verify SelK-mediated ubiquitin-dependent degradation of CDK4. Results SelK was found to be significantly upregulated in GB samples from short-term survivors (≤ 1 year) compared to those from long-term survivors (≥ 3 years), and its expression levels were negatively correlated with clinical prognosis. Knocking down of SelK expression reduced GB cell viability, induced G0/G1 phase arrest, and impaired the growth of transplanted glioma cells in nude mice. Down-regulation of SelK-induced ER stress leads to a reduction in the expression of SKP2 and an up-regulation of β-TrCP1 expression. Up-regulation of β-TrCP1, thereby accelerating the ubiquitin-dependent degradation of CDK4 and ultimately inhibiting the malignant proliferation of the GB cells. Conclusion This study discovered a significant increase in SelK expression in GB patients with poor prognosis, revealing a negative correlation between SelK expression and patient outcomes. Further mechanistic investigations revealed that SelK enhances the proliferation of GB cells by targeting the endoplasmic reticulum stress/SKP2/β-TrCP1/CDK4 axis. |
| Related Links | https://jeccr.biomedcentral.com/counter/pdf/10.1186/s13046-024-03157-x.pdf |
| Ending Page | 20 |
| Page Count | 20 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 17569966 |
| DOI | 10.1186/s13046-024-03157-x |
| Journal | Journal of Experimental & Clinical Cancer Research |
| Issue Number | 1 |
| Volume Number | 43 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2024-08-19 |
| Access Restriction | Open |
| Subject Keyword | Cancer Research Immunology Apoptosis Oncology Glioblastoma SelK Cell proliferation CDK4 Endoplasmic reticulum stress |
| Content Type | Text |
| Resource Type | Article |
| Subject | Oncology Cancer Research |
| Journal Impact Factor | 11.4/2023 |
| 5-Year Journal Impact Factor | 11.4/2023 |
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