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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Tuo, Decai Zhou, Peng Yan, Pu Cui, Hongguang Liu, Yang Wang, He Yang, Xiukun Liao, Wenbin Sun, Di Li, Xiaoying Shen, Wentao |
| Abstract | Background Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing. Results In this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus Potexvirus, family Alphaflexiviridae) that causes systemic infection with mild symptoms in cassava. CsCMV-CM was then modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome. The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months. Conclusions This CsCMV-based VIGS system provides a new tool for rapid and efficient gene function studies in cassava. |
| Related Links | https://plantmethods.biomedcentral.com/counter/pdf/10.1186/s13007-021-00775-w.pdf |
| Ending Page | 12 |
| Page Count | 12 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 17464811 |
| DOI | 10.1186/s13007-021-00775-w |
| Journal | Plant Methods |
| Issue Number | 1 |
| Volume Number | 17 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2021-07-12 |
| Access Restriction | Open |
| Subject Keyword | Plant Sciences Biological Techniques Cassava Cassava common mosaic virus Virus vector Virus-induced gene silencing |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Biotechnology Genetics |
| Journal Impact Factor | 4.7/2023 |
| 5-Year Journal Impact Factor | 5.6/2023 |
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