NDLI: Identification of the initial water-site and movement in Gleditsia sinensis seeds and its relation to seed coat structure

Content Provider	Springer Nature : BioMed Central
Author	Zhu, Mingwei Dai, Song Ma, Qiuyue Li, Shuxian
Abstract	Background Water uptake is essential for seed germination. However, Gleditsia sinensis seeds have a water-impermeable seed coat, which is beneficial for its adaption to the environment, but prohibits its germination without treatment. This feature may be associated with the structure of the seed coat. Thus, the aim of this research was to identify and describe the initial water uptake site and water movement and to determine the relationship between seed coat structure and water absorption. Results A water temperature of 80 °C was optimal to break the hardseededness of G. sinensis seeds. Scanning electron microscopy (SEM) images revealed that the seed coat consisted of a palisade layer and light line that can hinder water entry into the seed. Also, a structure of vascular bundles existed in the hilar region. Hot water treatment caused the tightly closed micropyle to open and the cavity beneath it expanded; the layer of palisade cells in the lens was raised. The embryo dye-tracking tests showed that the radicle tip was the initial region to be stained red. After staining for 24 h, the red-stained area on the vascular bundle side of cotyledon was more extensive than that on the other side. Further studies by MRI maps indicated that the micropyle was the initial site for water imbibition. Some water then migrated along the space between the seed coat and the endosperm to the chalazal; simultaneously, the rest of the water reached the embryonic axis and spread into cotyledons. The maps of 20–24 h showed that water was unevenly distributed within the cotyledons in a way that the edge parts were more hydrated than the center. Blocking tests showed that the hilar region was the initial and an important region during seed imbibition. The medial region and chalazal portion were capable of imbibing water when the hilar region was blocked, but water absorption was later and slower than that through the hilar region. Conclusion MRI technology provides a promising and non-invasive technique to identify the water gap and the path of water movement in the seed. Combined with the results of SEM, the relation between seed coat and its imbibition can be inferred.
Related Links	https://plantmethods.biomedcentral.com/counter/pdf/10.1186/s13007-021-00756-z.pdf
Ending Page	13
Page Count	13
Starting Page	1
File Format	HTM / HTML
ISSN	17464811
DOI	10.1186/s13007-021-00756-z
Journal	Plant Methods
Issue Number	1
Volume Number	17
Language	English
Publisher	BioMed Central
Publisher Date	2021-05-25
Access Restriction	Open
Subject Keyword	Plant Sciences Biological Techniques Hardseededness Leguminous seeds Hot water treatment Micropyle MRI
Content Type	Text
Resource Type	Article
Subject	Plant Science Biotechnology Genetics
Journal Impact Factor	4.7/2023
5-Year Journal Impact Factor	5.6/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina

Genome-wide analysis of microsatellite and sex-linked marker identification in Gleditsia sinensis

Germination behavior of cassia tora seeds in various pre-sowing treatment methods.

Towards portable MRI in the plant sciences

Full-length transcriptome characterization and comparative analysis of Gleditsia sinensis

The BELT and phenoSEED platforms: shape and colour phenotyping of seed samples

Use of ultrasonication to increase germination rates of Arabidopsis seeds

CT image segmentation of foxtail millet seeds based on semantic segmentation model VGG16-UNet

Identification and Characterization of Arabidopsis Seed Coat Mucilage Proteins

Identification of the initial water-site and movement in Gleditsia sinensis seeds and its relation to seed coat structure

Similar Documents

Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina

Genome-wide analysis of microsatellite and sex-linked marker identification in Gleditsia sinensis

Germination behavior of cassia tora seeds in various pre-sowing treatment methods.

Towards portable MRI in the plant sciences

Full-length transcriptome characterization and comparative analysis of Gleditsia sinensis

The BELT and phenoSEED platforms: shape and colour phenotyping of seed samples

Use of ultrasonication to increase germination rates of Arabidopsis seeds

CT image segmentation of foxtail millet seeds based on semantic segmentation model VGG16-UNet

Identification and Characterization of Arabidopsis Seed Coat Mucilage Proteins

Identification of the initial water-site and movement in Gleditsia sinensis seeds and its relation to seed coat structure