NDLI: Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species

Content Provider	Springer Nature : BioMed Central
Author	Laing, Eric D. Amaya, Moushimi Navaratnarajah, Chanakha K. Feng, Yan-Ru Cattaneo, Roberto Wang, Lin-Fa Broder, Christopher C.
Abstract	Background Hendra virus and Nipah virus are zoonotic viruses that have caused severe to fatal disease in livestock and human populations. The isolation of Cedar virus, a non-pathogenic virus species in the genus Henipavirus, closely-related to the highly pathogenic Hendra virus and Nipah virus offers an opportunity to investigate differences in pathogenesis and receptor tropism among these viruses. Methods We constructed full-length cDNA clones of Cedar virus from synthetic oligonucleotides and rescued two replication-competent, recombinant Cedar virus variants: a recombinant wild-type Cedar virus and a recombinant Cedar virus that expresses a green fluorescent protein from an open reading frame inserted between the phosphoprotein and matrix genes. Replication kinetics of both viruses and stimulation of the interferon pathway were characterized in vitro. Cellular tropism for ephrin-B type ligands was qualitatively investigated by microscopy and quantitatively by a split-luciferase fusion assay. Results Successful rescue of recombinant Cedar virus expressing a green fluorescent protein did not significantly affect virus replication compared to the recombinant wild-type Cedar virus. We demonstrated that recombinant Cedar virus stimulated the interferon pathway and utilized the established Hendra virus and Nipah virus receptor, ephrin-B2, but not ephrin-B3 to mediate virus entry. We further characterized virus-mediated membrane fusion kinetics of Cedar virus with the known henipavirus receptors ephrin-B2 and ephrin-B3. Conclusions The recombinant Cedar virus platform may be utilized to characterize the determinants of pathogenesis across the henipaviruses, investigate their receptor tropisms, and identify novel pan-henipavirus antivirals. Moreover, these experiments can be conducted safely under BSL-2 conditions.
Related Links	https://virologyj.biomedcentral.com/counter/pdf/10.1186/s12985-018-0964-0.pdf
Ending Page	12
Page Count	12
Starting Page	1
File Format	HTM / HTML
DOI	10.1186/s12985-018-0964-0
Journal	Virology Journal
Issue Number	1
Volume Number	15
Language	English
Publisher	BioMed Central
Publisher Date	2018-03-27
Access Restriction	Open
Subject Keyword	Virology Paramyxoviridae Cedar virus Henipaviruses Ephrin ligands Reverse genetics Recombinant virus Receptor tropism
Content Type	Text
Resource Type	Article
Subject	Virology Infectious Diseases
Journal Impact Factor	4/2023
5-Year Journal Impact Factor	3.8/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A Recombinant Chimeric Cedar Virus-Based Surrogate Neutralization Assay Platform for Pathogenic Henipaviruses.

Functional Analysis of the Fusion and Attachment Glycoproteins of Mojiang Henipavirus

Recombinant Hendra viruses expressing a reporter gene retain pathogenicity in ferrets

Functional Expression. Ephrin Receptor Tropism, and Heterotypic Functionality of the Attachment and Fusion Glycoproteins of Cedar Virus, a Newly Discovered Henipavirus

Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus.

Establishment of a Nipah virus rescue system

Detection of Nipah and Hendra Viruses Using Recombinant Human Ephrin B2 Capture Virus in Immunoassays

Cloning and rescue of the genome of Bombyx mori bidensovirus, and characterization of a recombinant virus

Capped antigenomic RNA transcript facilitates rescue of a plant rhabdovirus

Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species

Similar Documents

A Recombinant Chimeric Cedar Virus-Based Surrogate Neutralization Assay Platform for Pathogenic Henipaviruses.

Functional Analysis of the Fusion and Attachment Glycoproteins of Mojiang Henipavirus

Recombinant Hendra viruses expressing a reporter gene retain pathogenicity in ferrets

Functional Expression. Ephrin Receptor Tropism, and Heterotypic Functionality of the Attachment and Fusion Glycoproteins of Cedar Virus, a Newly Discovered Henipavirus

Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus.

Establishment of a Nipah virus rescue system

Detection of Nipah and Hendra Viruses Using Recombinant Human Ephrin B2 Capture Virus in Immunoassays

Cloning and rescue of the genome of Bombyx mori bidensovirus, and characterization of a recombinant virus

Capped antigenomic RNA transcript facilitates rescue of a plant rhabdovirus

Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species