NDLI: The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons

Content Provider	Springer Nature : BioMed Central
Author	Shi, Yao Li, Shuang Zhang, Haojie Zhu, Jianchun Che, Tongtong Yan, Bing Li, Jingxin Liu, Chuanyong
Abstract	Background The aim of the current study was to investigate the effect of macrophage polarization on the expression of oxytocin (OT) and the oxytocin receptor (OTR) in enteric neurons. Methods In this study, we used a classic colitis model and D-mannose model to observe the correlation between macrophage polarization and OT signalling system. In order to further demonstrate the effect of macrophages, we examined the expression of OT signalling system after depletion of macrophages. Results The data showed that, in vitro, following polarization of macrophages to the M1 type by LPS, the macrophage supernatant contained proinflammatory cytokines (IL-1β, IL-6 and TNF-α) that inhibited the expression of OT and OTR in cultured enteric neurons; following macrophage polarization to the M2 type by IL4, the macrophage supernatant contained anti-inflammatory cytokines (TGF-β) that promoted the expression of OT and OTR in cultured enteric neurons. Furthermore, M1 macrophages decreased the expression of the OT signalling system mainly through STAT3/NF-κB pathways in cultured enteric neurons; M2 macrophages increased the expression of the OT signalling system mainly through activation of Smad2/3 and inhibition of the expression of Peg3 in cultured enteric neurons. In a colitis model, we demonstrated that macrophages were polarized to the M1 type during the inflammatory phase, with significant decreased in the expression of OT and OTR. When macrophages were polarized to the M2 type during the recovery phase, OT and OTR expression increased significantly. In addition, we found that D-mannose increased the expression of OT and OTR through polarization of macrophages to the M2 type. Conclusions This is the first study to demonstrate that macrophage polarization differentially regulates the expression of OT and OTR in enteric neurons.
Related Links	https://jneuroinflammation.biomedcentral.com/counter/pdf/10.1186/s12974-021-02313-w.pdf
Ending Page	18
Page Count	18
Starting Page	1
File Format	HTM / HTML
ISSN	17422094
DOI	10.1186/s12974-021-02313-w
Journal	Journal of Neuroinflammation
Issue Number	1
Volume Number	18
Language	English
Publisher	BioMed Central
Publisher Date	2021-11-08
Access Restriction	Open
Subject Keyword	Neurosciences Neurology Neurobiology Immunology OT signalling system Macrophage polarization Proinflammatory and anti-inflammatory factors Neural-immune interactions
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology Cellular and Molecular Neuroscience Neurology
Journal Impact Factor	9.3/2023
5-Year Journal Impact Factor	9.8/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The immune system on the TRAIL of Alzheimer’s disease

TLR2 and TLR9 modulate enteric nervous system inflammatory responses to lipopolysaccharide

Anti-inflammatory activity of Wnt signaling in enteric nervous system: in vitro preliminary evidences in rat primary cultures

The immune response and aging in chronic inflammatory demyelinating polyradiculoneuropathy

Acetaminophen inhibits neuronal inflammation and protects neurons from oxidative stress

Neuroinflammation in inflammatory bowel disease

The involvement of mast cells in the irinotecan-induced enteric neurons loss and reactive gliosis

Targeted intracerebral delivery of the anti-inflammatory cytokine IL13 promotes alternative activation of both microglia and macrophages after stroke

Noninvasive ultrasound stimulation to treat myocarditis through splenic neuro-immune regulation

The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons

Similar Documents

The immune system on the TRAIL of Alzheimer’s disease

TLR2 and TLR9 modulate enteric nervous system inflammatory responses to lipopolysaccharide

Anti-inflammatory activity of Wnt signaling in enteric nervous system: in vitro preliminary evidences in rat primary cultures

The immune response and aging in chronic inflammatory demyelinating polyradiculoneuropathy

Acetaminophen inhibits neuronal inflammation and protects neurons from oxidative stress

Neuroinflammation in inflammatory bowel disease

The involvement of mast cells in the irinotecan-induced enteric neurons loss and reactive gliosis

Targeted intracerebral delivery of the anti-inflammatory cytokine IL13 promotes alternative activation of both microglia and macrophages after stroke

Noninvasive ultrasound stimulation to treat myocarditis through splenic neuro-immune regulation

The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons