NDLI: Coupling of nanostraws with diverse physicochemical perforation strategies for intracellular DNA delivery

Content Provider	Springer Nature : BioMed Central
Author	Jiang, Juan Liu, Jing Liu, Xinmin Xu, Xingyuan Liu, Zhengjie Huang, Shuang Huang, Xinshuo Yao, Chuanjie Wang, Xiafeng Chen, Yixin Chen, Hui-jiuan Wang, Ji Xie, Xi
Abstract	Effective intracellular DNA transfection is imperative for cell-based therapy and gene therapy. Conventional gene transfection methods, including biochemical carriers, physical electroporation and microinjection, face challenges such as cell type dependency, low efficiency, safety concerns, and technical complexity. Nanoneedle arrays have emerged as a promising avenue for improving cellular nucleic acid delivery through direct penetration of the cell membrane, bypassing endocytosis and endosome escape processes. Nanostraws (NS), characterized by their hollow tubular structure, offer the advantage of flexible solution delivery compared to solid nanoneedles. However, NS struggle to stably self-penetrate the cell membrane, resulting in limited delivery efficiency. Coupling with extra physiochemical perforation strategies is a viable approach to improve their performance. This study systematically compared the efficiency of NS coupled with polyethylenimine (PEI) chemical modification, mechanical force, photothermal effect, and electric field on cell membrane perforation and DNA transfection. The results indicate that coupling NS with PEI modification, mechanical force, photothermal effects provide limited enhancement effects. In contrast, NS-electric field coupling significantly improves intracellular DNA transfection efficiency. This work demonstrates that NS serve as a versatile platform capable of integrating various physicochemical strategies, while electric field coupling stands out as a form worthy of primary consideration for efficient DNA transfection.
Related Links	https://jnanobiotechnology.biomedcentral.com/counter/pdf/10.1186/s12951-024-02392-w.pdf
Ending Page	20
Page Count	20
Starting Page	1
File Format	HTM / HTML
ISSN	14773155
DOI	10.1186/s12951-024-02392-w
Journal	Journal of Nanobiotechnology
Issue Number	1
Volume Number	22
Language	English
Publisher	BioMed Central
Publisher Date	2024-03-26
Access Restriction	Open
Subject Keyword	Biotechnology Nanotechnology Molecular Medicine Nanostraw Intracellular delivery Physical field coupling Nano-electroporation Cell penetration
Content Type	Text
Resource Type	Article
Subject	Bioengineering Pharmaceutical Science Medicine Applied Microbiology and Biotechnology Biomedical Engineering Molecular Medicine Nanoscience and Nanotechnology
Journal Impact Factor	10.6/2023
5-Year Journal Impact Factor	11.4/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Engineering siRNA therapeutics: challenges and strategies

Correction: Nano-immunotherapy: overcoming delivery challenge of immune checkpoint therapy

Nanocarriers for intracellular delivery of proteins in biomedical applications: strategies and recent advances

Electroactive nanoinjection platform for intracellular delivery and gene silencing

Mucus interaction to improve gastrointestinal retention and pharmacokinetics of orally administered nano-drug delivery systems

Nano-drug delivery systems in wound treatment and skin regeneration

Physical stimuli-responsive DNA hydrogels: design, fabrication strategies, and biomedical applications

Exosome for mRNA delivery: strategies and therapeutic applications

Intraocular nano-microscale drug delivery systems for glaucoma treatment: design strategies and recent progress

Coupling of nanostraws with diverse physicochemical perforation strategies for intracellular DNA delivery

Similar Documents

Engineering siRNA therapeutics: challenges and strategies

Correction: Nano-immunotherapy: overcoming delivery challenge of immune checkpoint therapy

Nanocarriers for intracellular delivery of proteins in biomedical applications: strategies and recent advances

Electroactive nanoinjection platform for intracellular delivery and gene silencing

Mucus interaction to improve gastrointestinal retention and pharmacokinetics of orally administered nano-drug delivery systems

Nano-drug delivery systems in wound treatment and skin regeneration

Physical stimuli-responsive DNA hydrogels: design, fabrication strategies, and biomedical applications

Exosome for mRNA delivery: strategies and therapeutic applications

Intraocular nano-microscale drug delivery systems for glaucoma treatment: design strategies and recent progress

Coupling of nanostraws with diverse physicochemical perforation strategies for intracellular DNA delivery