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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Elton, Linzy Williams, Alan Ali, Shanom Heaphy, Jelena Pang, Vicky Commins, Liam O’Brien, Conor Yetiş, Özge Caine, Estelle Ward, Imogen Muzslay, Monika Yui, Samuel Karia, Kush Shore, Ellinor Rofael, Sylvia Mack, Damien J. F. McHugh, Timothy D. Wey, Emmanuel Q. |
| Abstract | Introduction Colonisation and infection with Carbapenem-resistant Enterobacterales (CRE) in healthcare settings poses significant risks, especially for vulnerable patients. Genomic analysis can be used to trace transmission routes, supporting antimicrobial stewardship and informing infection control strategies. Here we used genomic analysis to track the movement and transmission of CREs within clinical and environmental samples. Methods 25 isolates were cultured from clinical patient samples or swabs, that tested positive for OXA-48-like variants using the NG-Test® CARBA-5 test and whole genome sequenced (WGS) using Oxford Nanopore Technologies (ONT). 158 swabs and 52 wastewater samples were collected from the ward environment. 60 isolates (matching clinical isolate genera; Klebsiella, Enterobacter, Citrobacter and Escherichia) were isolated from the environmental samples using selective agar. Metagenomic sequencing was undertaken on 36 environmental wastewater and swab samples. Results 21/25 (84%) clinical isolates had > 1 blaOXA gene and 19/25 (76%) harboured > 1 blaNDM gene. Enterobacterales were most commonly isolated from environmental wastewater samples 27/52 (51.9%), then stick swabs 5/43 (11.6%) and sponge swabs 5/115 (4.3%). 11/60 (18%) environmental isolates harboured > 1 blaOXA gene and 1.9% (1/60) harboured blaNDM-1. blaOXA genes were found in 2/36 (5.5%) metagenomic environmental samples. Conclusions Potential for putative patient-patient and patient-ward transmission was shown. Metagenomic sampling needs optimization to improve sensitivity. |
| Related Links | https://ann-clinmicrob.biomedcentral.com/counter/pdf/10.1186/s12941-024-00762-8.pdf |
| Ending Page | 15 |
| Page Count | 15 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 14760711 |
| DOI | 10.1186/s12941-024-00762-8 |
| Journal | Annals of Clinical Microbiology and Antimicrobials |
| Issue Number | 1 |
| Volume Number | 23 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2024-12-20 |
| Access Restriction | Open |
| Subject Keyword | Medical Microbiology Infectious Diseases Drug Resistance Microbial Genetics and Genomics Biochemistry Parasitology Carbapenem-resistant Enterobacterales Antimicrobial resistance OXA48 NDM Plasmids Transmission Environment Hospital Metagenomics Oxford Nanopore CRE |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology (medical) Infectious Diseases |
| Journal Impact Factor | 4.6/2023 |
| 5-Year Journal Impact Factor | 5.3/2023 |
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