NDLI: Identification and validation of autophagy-related genes in primary open-angle glaucoma

Content Provider	Springer Nature : BioMed Central
Author	Xu, Wanjing Sun, Yuhao Zhao, Shuang Zhao, Jun Zhang, Juanmei
Abstract	Background As the most common type of glaucoma, the etiology of primary open-angle glaucoma (POAG) has not been unified. Autophagy may affect the occurrence and development of POAG, while the specific mechanism and target need to be further explored. Methods The GSE27276 dataset from the Gene Expression Omnibus (GEO) database and the autophagy gene set from the GeneCards database were selected to screen differentially expressed autophagy-related genes (DEARGs) of POAG. Hub DEARGs were selected by constructing protein-protein interaction (PPI) networks and utilizing GSE138125 dataset. Subsequently, immune cell infiltration analysis, genome-wide association study (GWAS) analysis, gene set enrichment analysis (GSEA) and other analyses were performed on the hub genes. Eventually, animal experiments were performed to verify the mRNA levels of the hub genes by quantitative real time polymerase chain reaction (qRT-PCR). Results A total of 67 DEARGs and 2 hub DEARGs, HSPA8 and RPL15, were selected. The hub genes were closely related to the level of immune cell infiltration. GWAS analysis confirmed that the causative regions of the 2 hub genes in glaucoma were on chromosome 11 and chromosome 3, respectively. GSEA illustrated that pathways enriched for highly expressed HSPA8 and RPL15 contained immunity, autophagy, gene expression and energy metabolism-related pathways. qRT-PCR confirmed that the expression of Hspa8 and Rpl15 in the rat POAG model was consistent with the results of bioinformatics analysis. Conclusions This study indicated that HSPA8 and RPL15 may affect the progression of POAG by regulating autophagy and provided new ideas for the pathogenesis and treatment of POAG.
Related Links	https://bmcmedgenomics.biomedcentral.com/counter/pdf/10.1186/s12920-023-01722-5.pdf
Ending Page	20
Page Count	20
Starting Page	1
File Format	HTM / HTML
ISSN	17558794
DOI	10.1186/s12920-023-01722-5
Journal	BMC Medical Genomics
Issue Number	1
Volume Number	16
Language	English
Publisher	BioMed Central
Publisher Date	2023-11-15
Access Restriction	Open
Subject Keyword	Human Genetics Microarrays Gene Expression Primary open-angle glaucoma Autophagy Differentially expressed genes Bioinformatics analysis RT-PCR HSPA8 RPL15
Content Type	Text
Resource Type	Article
Subject	Genetics (clinical) Genetics
Journal Impact Factor	2.1/2023
5-Year Journal Impact Factor	2.5/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Identification of differentially expressed genes associated with the pathogenesis of gastric cancer by bioinformatics analysis

Identification of key genes and pathways in atherosclerosis using integrated bioinformatics analysis

Identification of ferroptosis related markers by integrated bioinformatics analysis and In vitro model experiments in rheumatoid arthritis

Candidate genes identification and RNA-seq based pathway analysis associated with primary angle-closure glaucoma with cataract

Identification of genes and pathways involved in malignant pleural mesothelioma using bioinformatics methods

Identification of key genes and miRNAs related to polycystic ovary syndrome by comprehensive analysis of microarray

Comprehensive analysis of key genes and microRNAs in radioresistant nasopharyngeal carcinoma

Identification of key genes in calcific aortic valve disease via weighted gene co-expression network analysis

Bioinformatic identification of hub genes and related transcription factors in low shear stress treated endothelial cells

Identification and validation of autophagy-related genes in primary open-angle glaucoma

Similar Documents

Identification of differentially expressed genes associated with the pathogenesis of gastric cancer by bioinformatics analysis

Identification of key genes and pathways in atherosclerosis using integrated bioinformatics analysis

Identification of ferroptosis related markers by integrated bioinformatics analysis and In vitro model experiments in rheumatoid arthritis

Candidate genes identification and RNA-seq based pathway analysis associated with primary angle-closure glaucoma with cataract

Identification of genes and pathways involved in malignant pleural mesothelioma using bioinformatics methods

Identification of key genes and miRNAs related to polycystic ovary syndrome by comprehensive analysis of microarray

Comprehensive analysis of key genes and microRNAs in radioresistant nasopharyngeal carcinoma

Identification of key genes in calcific aortic valve disease via weighted gene co-expression network analysis

Bioinformatic identification of hub genes and related transcription factors in low shear stress treated endothelial cells

Identification and validation of autophagy-related genes in primary open-angle glaucoma