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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Cong, Feng Zhu, Yujun Wang, Jing Lian, Yuexiao Liu, Xiangnan Xiao, Li Huang, Ren Zhang, Yu Chen, Meili Guo, Pengju |
| Abstract | Background Chicken anemia virus (CAV), avian reovirus (ARV), infectious bursal disease virus (IBDV), Marek’s disease virus (MDV) and reticuloendotheliosis virus (REV) all cause immunosuppressive disease in birds through vertical or horizontal transmission. Mixed infections with these immunosuppressive pathogens lead to atypical clinical signs and obstruct accurate diagnoses and epidemiological investigations. Therefore, it is essential to develop a high-throughput assay for the simultaneous detection of these immunosuppressive viruses with high specificity and sensitivity. The aim of this study was to establish a novel method using a RT-PCR assay combined with fluorescence labeled polystyrene bead microarray (multiplex xTAG assay) to detect single or mixed viral infections. Results The results showed that the established xTAG assay had no nonspecific reactions with avian influenza virus (AIV), infectious bronchitis virus (IBV), newcastle disease virus (NDV), infectious laryngotracheitis virus (ILTV), Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). The limit of detection was 1.0 × 103 copies/μL for IBDV and 1.0 × 102copies/μL for the other four viruses. Ninety field samples were tested and the results were confirmed using conventional RT-PCR methods. The detection results of these two methods were 100% consistent. The established multiplex xTAG assay allows a high throughput and simultaneous detection of five chicken immunosuppressive viruses. Conclusion The multiplex xTAG assay has been showed to be an additional tool for molecular epidemiology studies of five chicken immunosuppressive viruses in the poultry industry. |
| Related Links | https://bmcvetres.biomedcentral.com/counter/pdf/10.1186/s12917-018-1663-1.pdf |
| Ending Page | 8 |
| Page Count | 8 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 17466148 |
| DOI | 10.1186/s12917-018-1663-1 |
| Journal | BMC Veterinary Research |
| Issue Number | 1 |
| Volume Number | 14 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2018-11-15 |
| Access Restriction | Open |
| Subject Keyword | Veterinary Medicine Veterinary Science Zoology Transgenics Simultaneous detection Multiplex xTAG assay Chicken anemia virus Avian reovirus Chicken immunosuppressive viruses Veterinary Medicine/Veterinary Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary Medicine |
| Journal Impact Factor | 2.3/2023 |
| 5-Year Journal Impact Factor | 2.6/2023 |
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