NDLI: Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission

Content Provider	Springer Nature : BioMed Central
Author	Bower, Danielle V Lee, Hyung-Kook Lansford, Rusty Zinn, Kai Warburton, David Fraser, Scott E Jesudason, Edwin C
Abstract	Background Parasympathetic signaling has been inferred to regulate epithelial branching as well as organ regeneration and tumor development. However, the relative contribution of local nerve contact versus secreted signals remains unclear. Here, we show a conserved (vertebrates to invertebrates) requirement for intact local nerves in airway branching, persisting even when cholinergic neurotransmission is blocked. Results In the vertebrate lung, deleting enhanced green fluorescent protein (eGFP)-labeled intrinsic neurons using a two-photon laser leaves adjacent cells intact, but abolishes branching. Branching is unaffected by similar laser power delivered to the immediately adjacent non-neural mesodermal tissue, by blocking cholinergic receptors or by blocking synaptic transmission with botulinum toxin A. Because adjacent vasculature and epithelial proliferation also contribute to branching in the vertebrate lung, the direct dependence on nerves for airway branching was tested by deleting neurons in Drosophila embryos. A specific deletion of neurons in the Drosophila embryo by driving cell-autonomous RicinA under the pan-neuronal elav enhancer perturbed Drosophila airway development. This system confirmed that even in the absence of a vasculature or epithelial proliferation, airway branching is still disrupted by neural lesioning. Conclusions Together, this shows that airway morphogenesis requires local innervation in vertebrates and invertebrates, yet neurotransmission is dispensable. The need for innervation persists in the fly, wherein adjacent vasculature and epithelial proliferation are absent. Our novel, targeted laser ablation technique permitted the local function of parasympathetic innervation to be distinguished from neurotransmission.
Related Links	https://bmcbiol.biomedcentral.com/counter/pdf/10.1186/s12915-014-0092-2.pdf
Ending Page	13
Page Count	13
Starting Page	1
File Format	HTM / HTML
ISSN	17417007
DOI	10.1186/s12915-014-0092-2
Journal	BMC Biology
Issue Number	1
Volume Number	12
Language	English
Publisher	BioMed Central
Publisher Date	2014-11-11
Access Restriction	Open
Subject Keyword	Life Sciences Airway branching Lung morphogenesis Lung development Tracheal branching Trachea development Innervation Denervation Neurotransmission Parasympathetic Laser ablation
Content Type	Text
Resource Type	Article
Subject	Ecology, Evolution, Behavior and Systematics Structural Biology Biochemistry, Genetics and Molecular Biology Plant Science Biotechnology Physiology Agricultural and Biological Sciences Cell Biology Developmental Biology
Journal Impact Factor	4.4/2023
5-Year Journal Impact Factor	5.4/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission

Smooth Muscle Differentiation Is Essential for Airway Size, Tracheal Cartilage Segmentation, but Dispensable for Epithelial Branching

Conserved Mechanisms in the Formation of the Airways and Alveoli of the Lung

Myosin II activity is not required for Drosophila tracheal branching morphogenesis

Lung mesenchymal expression of Sox9plays a critical role in tracheal development

Hmga2is required for canonical WNT signaling during lung development

Editorial: Branching Morphogenesis During Embryonic Lung Development

Mesenchymal adenomatous polyposis coli plays critical and diverse roles in regulating lung development

Comparative molecular developmental aspects of the mammalian- and the avian lungs, and the insectan tracheal system by branching morphogenesis: recent advances and future directions

Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission

Similar Documents

Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission

Smooth Muscle Differentiation Is Essential for Airway Size, Tracheal Cartilage Segmentation, but Dispensable for Epithelial Branching

Conserved Mechanisms in the Formation of the Airways and Alveoli of the Lung

Myosin II activity is not required for Drosophila tracheal branching morphogenesis

Lung mesenchymal expression of Sox9plays a critical role in tracheal development

Hmga2is required for canonical WNT signaling during lung development

Editorial: Branching Morphogenesis During Embryonic Lung Development

Mesenchymal adenomatous polyposis coli plays critical and diverse roles in regulating lung development

Comparative molecular developmental aspects of the mammalian- and the avian lungs, and the insectan tracheal system by branching morphogenesis: recent advances and future directions

Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission