NDLI: The activation of pyrin domain-containing-3 inflammasome depends on lipopolysaccharide from Porphyromonas gingivalis and extracellular adenosine triphosphate in cultured oral epithelial cells

Content Provider	Springer Nature : BioMed Central
Author	Guo, Wei Wang, Peng Liu, Zhonghao Yang, Pishan Ye, Ping
Abstract	Background Gingival epithelial cells are the major population of the gingival tissue, acting as the front-line defense against microbial intrusion and regulating the homeostasis of the periodontal tissue in health and disease via NLR family pyrin domain-containing-3 (NLRP3) inflammasome, which recognizes pathogen- and danger-associated molecular patterns (PAMPs and DAMPs). The aim of this study was to determine whether the activation of NLRP3 inflammasome depends on infection with the periodontal pathogen Porphyromonas gingivalis (P. gingivalis), or stimulation with P. gingivalis lipopolysaccharide (LPS), and/or extracellular adenosine triphosphate (ATP). Methods An oral epithelial cell line was treated with P. gingivalis, P. gingivalis LPS and ATP. The gene and protein expression of NLRP3 inflammasome components were quantified by real time RT-PCR and immunoblots. Production of IL-1β and IL-18 was measured by ELISA. Results There was no increase in NLRP3 inflammasome gene expression after P. gingivalis infection unless pre-stimulated by ATP. Obvious increases of NLRP3 inflammasome gene expression was observed after P. gingivalis LPS stimulation, even pre-stimulated by ATP at 2 h. Conclusions The findings indicate that the activation of NLRP3 inflammasome does not rely on P. gingivalis infection, unless stimulated by P. gingivalis LPS and/or extracellular ATP, suggesting diverse signaling pathways are involved in the host immune response.
Related Links	https://bmcoralhealth.biomedcentral.com/counter/pdf/10.1186/s12903-015-0115-6.pdf
Ending Page	11
Page Count	11
Starting Page	1
File Format	HTM / HTML
ISSN	14726831
DOI	10.1186/s12903-015-0115-6
Journal	BMC Oral Health
Issue Number	1
Volume Number	15
Language	English
Publisher	BioMed Central
Publisher Date	2015-10-29
Access Restriction	Open
Subject Keyword	Dentistry Oral and Maxillofacial Surgery Porphyromonas gingivalis (P. gingivalis) P. gingivalis LPS Extracellular adenosine triphosphate (ATP) NLRP3 inflammasome
Content Type	Text
Resource Type	Article
Subject	Dentistry
Journal Impact Factor	2.6/2023
5-Year Journal Impact Factor	3.2/2023

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