NDLI: A tight balance of Karyopherin β1 expression is required in cervical cancer cells

Content Provider	Springer Nature : BioMed Central
Author	Carden, Sarah van der Watt, Pauline Chi, Alicia Ajayi-Smith, Aderonke Hadley, Katie Leaner, Virna D.
Abstract	Background Karyopherin β1 (Kpnβ1) is the main nuclear import protein involved in the transport of cargoes from the cytoplasm into the cell nucleus. Previous research has found Kpnβ1 to be significantly overexpressed in cervical cancer and other cancer tissues, and further studies showed that inhibition of Kpnβ1 expression by siRNA resulted in cancer cell death, while non-cancer cells were minimally affected. These results suggest that Kpnβ1 has potential as an anticancer therapeutic target, thus warranting further research into the association between Kpnβ1 expression and cancer progression. Here, the biological effects associated with Kpnβ1 overexpression were investigated in order to further elucidate the relationship between Kpnβ1 and the cancer phenotype. Methods To evaluate the effect of Kpnβ1 overexpression on cell biology, cell proliferation, cell cycle, cell morphology and cell adhesion assays were performed. To determine whether Kpnβ1 overexpression influences cell sensitivity to chemotherapeutic agents like Cisplatin, cell viability assays were performed. Expression levels of key proteins were analysed by Western blot analysis. Results Our data revealed that Kpnβ1 overexpression, above that which was already detected in cancer cells, resulted in reduced proliferation of cervical cancer cells. Likewise, normal epithelial cells showed reduced proliferation after Kpnβ1 overxpression. Reduced cancer cell proliferation was associated with a delay in cell cycle progression, as well as changes in the morphology and adhesion properties of cells. Additionally, Kpnβ1 overexpressing HeLa cells exhibited increased sensitivity to cisplatin, as shown by decreased cell viability and increased apoptosis, where p53 and p21 inhibition reduced and enhanced cell sensitivity to Cisplatin, respectively. Conclusions Overall, our results suggest that a tight balance of Kpnβ1 expression is required for cellular function, and that perturbation of this balance results in negative effects associated with a variety of biological processes.
Related Links	https://bmccancer.biomedcentral.com/counter/pdf/10.1186/s12885-018-5044-8.pdf
Ending Page	15
Page Count	15
Starting Page	1
File Format	HTM / HTML
ISSN	14712407
DOI	10.1186/s12885-018-5044-8
Journal	BMC Cancer
Issue Number	1
Volume Number	18
Language	English
Publisher	BioMed Central
Publisher Date	2018-11-16
Access Restriction	Open
Subject Keyword	Cancer Research Oncology Surgical Oncology Health Promotion and Disease Prevention Biomedicine Medicine Public Health Karypherin β1 Overexpression Cancer biology Medicine/Public Health
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology Genetics
Journal Impact Factor	3.4/2023
5-Year Journal Impact Factor	3.8/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

HER2 expression in cervical cancer as a potential therapeutic target

microRNA-944 overexpression is a biomarker for poor prognosis of advanced cervical cancer

Overexpression of iASPP is required for autophagy in response to oxidative stress in choriocarcinoma

MiR-23b controls ALDH1A1 expression in cervical cancer stem cells

Overexpression of cathepsin f, matrix metalloproteinases 11 and 12 in cervical cancer

Nuclear expression of Rac1 in cervical premalignant lesions and cervical cancer cells

URG4 overexpression is correlated with cervical cancer progression and poor prognosis in patients with early-stage cervical cancer

Erratum to: Overexpression of cathepsin f, matrix metalloproteinases 11 and 12 in cervical cancer

Apoptosis inhibitor-5 overexpression is associated with tumor progression and poor prognosis in patients with cervical cancer

A tight balance of Karyopherin β1 expression is required in cervical cancer cells

Similar Documents

HER2 expression in cervical cancer as a potential therapeutic target

microRNA-944 overexpression is a biomarker for poor prognosis of advanced cervical cancer

Overexpression of iASPP is required for autophagy in response to oxidative stress in choriocarcinoma

MiR-23b controls ALDH1A1 expression in cervical cancer stem cells

Overexpression of cathepsin f, matrix metalloproteinases 11 and 12 in cervical cancer

Nuclear expression of Rac1 in cervical premalignant lesions and cervical cancer cells

URG4 overexpression is correlated with cervical cancer progression and poor prognosis in patients with early-stage cervical cancer

Erratum to: Overexpression of cathepsin f, matrix metalloproteinases 11 and 12 in cervical cancer

Apoptosis inhibitor-5 overexpression is associated with tumor progression and poor prognosis in patients with cervical cancer

A tight balance of Karyopherin β1 expression is required in cervical cancer cells