NDLI: MYB transcription factors, their regulation and interactions with non-coding RNAs during drought stress in Brassica juncea

Content Provider	Springer Nature : BioMed Central
Author	Balhara, Rinku Verma, Deepika Kaur, Ravneet Singh, Kashmir
Abstract	Background Brassica juncea (L.) Czern is an important oilseed crop affected by various abiotic stresses like drought, heat, and salt. These stresses have detrimental effects on the crop’s overall growth, development and yield. Various Transcription factors (TFs) are involved in regulation of plant stress response by modulating expression of stress-responsive genes. The myeloblastosis (MYB) TFs is one of the largest families of TFs associated with various developmental and biological processes such as plant growth, secondary metabolism, stress response etc. However, MYB TFs and their regulation by non-coding RNAs (ncRNAs) in response to stress have not been studied in B. juncea. Thus, we performed a detailed study on the MYB TF family and their interactions with miRNAs and Long non coding RNAs. Results Computational investigation of genome and proteome data presented a comprehensive picture of the MYB genes and their protein architecture, including intron-exon organisation, conserved motif analysis, R2R3 MYB DNA-binding domains analysis, sub-cellular localization, protein-protein interaction and chromosomal locations. Phylogenetically, BjuMYBs were further classified into different subclades on the basis of topology and classification in Arabidopsis. A total of 751 MYBs were identified in B. juncea corresponding to 297 1R-BjuMYBs, 440 R2R3-BjuMYBs, 12 3R-BjuMYBs, and 2 4R-BjuMYBs types. We validated the transcriptional profiles of nine selected BjuMYBs under drought stress through RT-qPCR. Promoter analysis indicated the presence of drought-responsive cis-regulatory components. Additionally, the miRNA-MYB TF interactions was also studied, and most of the microRNAs (miRNAs) that target BjuMYBs were involved in abiotic stress response and developmental processes. Regulatory network analysis and expression patterns of lncRNA-miRNA-MYB indicated that selected long non-coding RNAs (lncRNAs) acted as strong endogenous target mimics (eTMs) of the miRNAs regulated expression of BjuMYBs under drought stress. Conclusions The present study has established preliminary groundwork of MYB TFs and their interaction with ncRNAs in B. juncea and it will help in developing drought- tolerant Brassica crops.
Related Links	https://bmcplantbiol.biomedcentral.com/counter/pdf/10.1186/s12870-024-05736-8.pdf
Ending Page	18
Page Count	18
Starting Page	1
File Format	HTM / HTML
ISSN	14712229
DOI	10.1186/s12870-024-05736-8
Journal	BMC Plant Biology
Issue Number	1
Volume Number	24
Language	English
Publisher	BioMed Central
Publisher Date	2024-10-24
Access Restriction	Open
Subject Keyword	Plant Sciences Agriculture Tree Biology MYB domain Cis-regulatory elements Brassica juncea lncRNAs Abiotic stress miRNA targets Expression profile
Content Type	Text
Resource Type	Article
Subject	Plant Science
Journal Impact Factor	4.3/2023
5-Year Journal Impact Factor	5.2/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Profile of small interfering rnas from french bean phaseolus vulgaris under abiotic stress conditions.

Molecular characterization of 60 isolated wheat MYB genes and analysis of their expression during abiotic stress

Genome wide identification of BjSWEET gene family and drought response analysis of BjSWEET12 and BjSWEET17 genes in Brassica juncea

Identification of novel microRNAs in Hevea brasiliensisand computational prediction of their targets

Global insights into high temperature and drought stress regulated genes by RNA-Seq in economically important oilseed crop Brassica juncea

Potential role of the regulatory miR1119-MYC2 module in wheat (Triticum aestivum L.) drought tolerance

The Characters of Non-Coding RNAs and Their Biological Roles in Plant Development and Abiotic Stress Response

PASmiR: a literature-curated database for miRNA molecular regulation in plant response to abiotic stress

Long non-coding RNAs: emerging players regulating plant abiotic stress response and adaptation

MYB transcription factors, their regulation and interactions with non-coding RNAs during drought stress in Brassica juncea

Similar Documents

Profile of small interfering rnas from french bean phaseolus vulgaris under abiotic stress conditions.

Molecular characterization of 60 isolated wheat MYB genes and analysis of their expression during abiotic stress

Genome wide identification of BjSWEET gene family and drought response analysis of BjSWEET12 and BjSWEET17 genes in Brassica juncea

Identification of novel microRNAs in Hevea brasiliensisand computational prediction of their targets

Global insights into high temperature and drought stress regulated genes by RNA-Seq in economically important oilseed crop Brassica juncea

Potential role of the regulatory miR1119-MYC2 module in wheat (Triticum aestivum L.) drought tolerance

The Characters of Non-Coding RNAs and Their Biological Roles in Plant Development and Abiotic Stress Response

PASmiR: a literature-curated database for miRNA molecular regulation in plant response to abiotic stress

Long non-coding RNAs: emerging players regulating plant abiotic stress response and adaptation

MYB transcription factors, their regulation and interactions with non-coding RNAs during drought stress in Brassica juncea