NDLI: Constitutive and induced insect resistance in RNAi-mediated ultra-low gossypol cottonseed cotton

Content Provider	Springer Nature : BioMed Central
Author	Hagenbucher, Steffen Eisenring, Michael Meissle, Michael Rathore, Keerti S. Romeis, Jörg
Abstract	Background Besides fibers, cotton plants also produce a large amount of seeds with a high oil and protein content. The use of these seeds is restricted by their high contents of the terpenoid gossypol, which is harmful to humans and livestock. Using a genetic engineering approach, “Ultra-low gossypol cottonseed” (ULGCS) plants were produced by knocking down an enzyme that catalyzes the formation of a precursor of gossypol. This was accomplished via RNAi-mediated silencing of the target gene using a seed-specific α-globulin promotor. Since gossypol is also a crucial defense mechanism against leaf-feeding herbivores, ULGCS plants might possess lower herbivore resistance than non-engineered plants. Therefore, we tested the constitutive and inducible direct insect resistance of two ULGCS cotton lines against the African cotton leafworm, Spodoptera littoralis. Result The herbivore was equally affected by both ULGCS lines and the control (Coker 312) line when feeding on fully expanded true leaves from undamaged plants and plants induced by jasmonic acid. When plants were induced by caterpillar-damage, however, S. littoralis larvae performed better on the ULGCS plants. Terpenoid analyses revealed that the ULGCS lines were equally inducible as the control plants. Levels of terpenoids were always lower in one of the two lines. In the case of cotyledons, caterpillars performed better on ULGCS cotton than on conventional cotton. This was likely caused by reduced levels of gossypol in ULGCS cotyledons. Conclusion Despite those effects, the insect resistance of ULGSC cotton can be considered as largely intact and the plants may, therefore, be an interesting alternative to conventional cotton varieties.
Related Links	https://bmcplantbiol.biomedcentral.com/counter/pdf/10.1186/s12870-019-1921-9.pdf
Ending Page	10
Page Count	10
Starting Page	1
File Format	HTM / HTML
ISSN	14712229
DOI	10.1186/s12870-019-1921-9
Journal	BMC Plant Biology
Issue Number	1
Volume Number	19
Language	English
Publisher	BioMed Central
Publisher Date	2019-07-18
Access Restriction	Open
Subject Keyword	Plant Sciences Agriculture Tree Biology Gossypium hirsutum Spodoptera littoralis ULGCS Glandless cotton Gossypol Genetically modified crops TAM66274
Content Type	Text
Resource Type	Article
Subject	Plant Science
Journal Impact Factor	4.3/2023
5-Year Journal Impact Factor	5.2/2023

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