NDLI: Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content

Content Provider	Springer Nature : BioMed Central
Author	Gu, Jianwei Hou, Dalin Li, Yonghong Chao, Hongbo Zhang, Kai Wang, Hao Xiang, Jun Raboanatahiry, Nadia Wang, Baoshan Li, Maoteng
Abstract	Background Rapeseed (Brassica napus, B. napus) is an important oil seed crop in the world. Previous studies showed that seed germination vigor might be correlated with seed oil content in B. napus, but the regulation mechanism for seed germination has not yet been explained clearly. Dissecting the regulation mechanism of seed germination and germination vigor is necessary. Results Here, proteomic and genomic approaches were used to analyze the germination process in B. napus seeds with different oil content. The identification of 165 differentially expressed proteins (DEPs) in the germinating seeds of B. napus with high and low oil content was accomplished by two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE). The comparative proteomic results revealed that seeds with high oil content had higher metabolic activity, especially for sulfur amino acid metabolism. Thirty-one unique genes were shown to be significantly changed during germination between the seeds with high and low oil content, and thirteen of these genes were located within the confidence interval of germination-related quantitative trait locus (QTLs), which might play an important role in regulating seed germination vigor. Conclusions The present results are of importance for the understanding of the regulation mechanism for seed germination vigor in B. napus.
Related Links	https://bmcplantbiol.biomedcentral.com/counter/pdf/10.1186/s12870-018-1624-7.pdf
Ending Page	20
Page Count	20
Starting Page	1
File Format	HTM / HTML
ISSN	14712229
DOI	10.1186/s12870-018-1624-7
Journal	BMC Plant Biology
Issue Number	1
Volume Number	19
Language	English
Publisher	BioMed Central
Publisher Date	2019-01-11
Access Restriction	Open
Subject Keyword	Plant Sciences Agriculture Tree Biology Brassica Seed germination vigor 2D-DIGE QTL analysis
Content Type	Text
Resource Type	Article
Subject	Plant Science
Journal Impact Factor	4.3/2023
5-Year Journal Impact Factor	5.2/2023

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