NDLI: Nogo receptor is involved in the adhesion of dendritic cells to myelin

Content Provider	Springer Nature : BioMed Central
Author	McDonald, Claire L Steinbach, Karin Kern, Florian Schweigreiter, Rüdiger Martin, Roland Bandtlow, Christine E Reindl, Markus
Abstract	Background Nogo-66 receptor NgR1 and its structural homologue NgR2 are binding proteins for a number of myelin-associated inhibitory factors. After neuronal injury, these inhibitory factors are responsible for preventing axonal outgrowth via their interactions with NgR1 and NgR2 expressed on neurons. In vitro, cells expressing NgR1/2 are inhibited from adhering to and spreading on a myelin substrate. Neuronal injury also results in the presence of dendritic cells (DCs) in the central nervous system, where they can come into contact with myelin debris. The exact mechanisms of interaction of immune cells with CNS myelin are, however, poorly understood. Methods Human DCs were differentiated from peripheral blood monocytes and mouse DCs were differentiated from wild type and NgR1/NgR2 double knockout bone marrow precursors. NgR1 and NgR2 expression were determined with quantitative real time PCR and immunoblot, and adhesion of cells to myelin was quantified. Results We demonstrate that human immature myeloid DCs express NgR1 and NgR2, which are then down-regulated upon maturation. Human mature DCs also adhere to a much higher extent to a myelin substrate than immature DCs. We observe the same effect when the cells are plated on Nogo-66-His (binding peptide for NgR1), but not on control proteins. Mature DCs taken from Ngr1/2 knockout mice adhere to a much higher extent to myelin compared to wild type mouse DCs. In addition, Ngr1/2 knockout had no effect on in vitro DC differentiation or phenotype. Conclusions These results indicate that a lack of NgR1/2 expression promotes the adhesion of DCs to myelin. This interaction could be important in neuroinflammatory disorders such as multiple sclerosis in which peripheral immune cells come into contact with myelin debris.
Related Links	https://jneuroinflammation.biomedcentral.com/counter/pdf/10.1186/1742-2094-8-113.pdf
Ending Page	12
Page Count	12
Starting Page	1
File Format	HTM / HTML
ISSN	17422094
DOI	10.1186/1742-2094-8-113
Journal	Journal of Neuroinflammation
Issue Number	1
Volume Number	8
Language	English
Publisher	BioMed Central
Publisher Date	2011-09-09
Access Restriction	Open
Subject Keyword	Neurosciences Neurology Neurobiology Immunology Nogo receptor NgR1 NgR2 Nogo-66 myelin associated glycoprotein MAG myelin dendritic cells
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology Cellular and Molecular Neuroscience Neurology
Journal Impact Factor	9.3/2023
5-Year Journal Impact Factor	9.8/2023

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