NDLI: Swarming and complex pattern formation in Paenibacillus vortex studied by imaging and tracking cells

Content Provider	Springer Nature : BioMed Central
Author	Ingham, Colin J Jacob, Eshel Ben
Abstract	Background Swarming motility allows microorganisms to move rapidly over surfaces. The Gram-positive bacterium Paenibacillus vortex exhibits advanced cooperative motility on agar plates resulting in intricate colonial patterns with geometries that are highly sensitive to the environment. The cellular mechanisms that underpin the complex multicellular organization of such a simple organism are not well understood. Results Swarming by P. vortex was studied by real-time light microscopy, by in situ scanning electron microscopy and by tracking the spread of antibiotic-resistant cells within antibiotic-sensitive colonies. When swarming, P. vortex was found to be peritrichously flagellated. Swarming by the curved cells of P. vortex occurred on an extremely wide range of media and agar concentrations (0.3 to 2.2% w/v). At high agar concentrations (> 1% w/v) rotating colonies formed that could be detached from the main mass of cells by withdrawal of cells into the latter. On lower percentage agars, cells moved in an extended network composed of interconnected \"snakes\" with short-term collision avoidance and sensitivity to extracts from swarming cells. P. vortex formed single Petri dish-wide \"supercolonies\" with a colony-wide exchange of motile cells. Swarming cells were coupled by rapidly forming, reversible and non-rigid connections to form a loose raft, apparently connected via flagella. Inhibitors of swarming (p-Nitrophenylglycerol and Congo Red) were identified. Mitomycin C was used to trigger filamentation without inhibiting growth or swarming; this facilitated dissection of the detail of swarming. Mitomycin C treatment resulted in malcoordinated swarming and abortive side branch formation and a strong tendency by a subpopulation of the cells to form minimal rotating aggregates of only a few cells. Conclusion P. vortex creates complex macroscopic colonies within which there is considerable reflux and movement and interaction of cells. Cell shape, flagellation, the aversion of cell masses to fuse and temporary connections between proximate cells to form rafts were all features of the swarming and rotation of cell aggregates. Vigorous vortex formation was social, i.e. required > 1 cell. This is the first detailed examination of the swarming behaviour of this bacterium at the cellular level.
Related Links	https://bmcmicrobiol.biomedcentral.com/counter/pdf/10.1186/1471-2180-8-36.pdf
Ending Page	16
Page Count	16
Starting Page	1
File Format	HTM / HTML
ISSN	14712180
DOI	10.1186/1471-2180-8-36
Journal	BMC Microbiology
Issue Number	1
Volume Number	8
Language	English
Publisher	BioMed Central
Publisher Date	2008-02-25
Access Restriction	Open
Subject Keyword	Microbiology Biological Microscopy Mycology Parasitology Virology Life Sciences Surfactin Motile Cell Extracellular Material Vortex Cell Detergent Activity
Content Type	Text
Resource Type	Article
Subject	Microbiology Microbiology (medical)
Journal Impact Factor	4/2023
5-Year Journal Impact Factor	4.6/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Swarming and complex pattern formation in Paenibacillus vortex studied by imaging and tracking cells

Rhamnolipids and surfactin inhibit the growth or formation of oral bacterial biofilm

Surfactin inhibits enterococcal biofilm formation via interference with pilus and exopolysaccharide biosynthesis

Roles of curli, cellulose and BapA in Salmonella biofilm morphology studied by atomic force microscopy

Identification of novel surfactin derivatives from NRPS modification of Bacillus subtilis and its antifungal activity

Lipopeptide mediated biocontrol activity of endophytic Bacillus subtilis against fungal phytopathogens

Pellicle formation in Shewanella oneidensis

Biofilm formation assessment in Sinorhizobium meliloti reveals interlinked control with surface motility

Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403

Swarming and complex pattern formation in Paenibacillus vortex studied by imaging and tracking cells

Similar Documents

Swarming and complex pattern formation in Paenibacillus vortex studied by imaging and tracking cells

Rhamnolipids and surfactin inhibit the growth or formation of oral bacterial biofilm

Surfactin inhibits enterococcal biofilm formation via interference with pilus and exopolysaccharide biosynthesis

Roles of curli, cellulose and BapA in Salmonella biofilm morphology studied by atomic force microscopy

Identification of novel surfactin derivatives from NRPS modification of Bacillus subtilis and its antifungal activity

Lipopeptide mediated biocontrol activity of endophytic Bacillus subtilis against fungal phytopathogens

Pellicle formation in Shewanella oneidensis

Biofilm formation assessment in Sinorhizobium meliloti reveals interlinked control with surface motility

Scattered migrating colony formation in the filamentous cyanobacterium, Pseudanabaena sp. NIES-4403

Swarming and complex pattern formation in Paenibacillus vortex studied by imaging and tracking cells