NDLI: A high efficiency in vitro regeneration protocol and clonal uniformity analysis in Hypericum hookerianum Wight & Arn., a lesser known plant of ethnomedicinal and economic importance

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A high efficiency in vitro regeneration protocol and clonal uniformity analysis in Hypericum hookerianum Wight & Arn., a lesser known plant of ethnomedicinal and economic importance

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Brazilian Journal of Botany : Volume 38

Brazilian Journal of Botany : Volume 37

Brazilian Journal of Botany : Volume 36

A high efficiency in vitro regeneration protocol and clonal uniformity analysis in Hypericum hookerianum Wight & Arn., a lesser known plant of ethnomedicinal and economic importance

Content Provider	Springer Nature Link
Author	Seeni, Sooriamuthu Pushparaj, Selvadoss Pradeep Joseph Varghese, Reji Bayyapureddy, Anusha
Copyright Year	2015
Abstract	Hypericum hookerianum (Hypericaceae), a critically endangered plant of Western Ghats, India, has acquired significant importance due to its medicinal implications and ornamental flowers. This species is under severe anthropogenic pressure due to urbanization, tourism, and plantation activities taking place in their natural habitat. A clonal propagation strategy is standardized in this species which offers an opportunity for a stable production of active metabolites from this species as well as their conservation. The study deals with the optimization of axillary bud proliferation using nodal explants followed by genetic stability analysis of regenerants. Maximum number of shoots (3.66) was observed on the Murashige and Skoog (MS) medium supplemented with Kinetin (2.325 µM) with 85 % shoot multiplication frequency. In vitro grown shoots were rooted best in 1/2 MS medium supplemented with indole-3-butyric acid (2.45 µM) with an average of 6.8 ± 0.79 roots/shoot and 95.5 % rooting frequency. Plantlets were acclimatized best (90 %) in a mixture of sterile sand and farmyard manure (3:1). Micropropagated plants were subjected to random amplified polymorphic DNA analysis and phytochemical analysis to confirm their clonal stability. In RAPD analysis, 1032 amplicons were collectively generated which were monomorphic and similar to the mother plant. The comparable major phytochemical constituents in regenerants and mother plants together with genetic uniformity data obtained from RAPD analysis confirmed clonal fidelity of the regenerants. Findings in this study are the first report on micropropagation and assessment of genetic stability of micropropagated plantlets in H. hookerianum which suggests that in vitro axillary shoot proliferation can effectively be used as a tool for propagation and conservation of H. hookerianum.
Starting Page	377
Ending Page	386
Page Count	10
File Format	PDF
ISSN	01008404
Journal	Brazilian Journal of Botany
Volume Number	39
Issue Number	1
e-ISSN	18069959
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-08-23
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Systematics/Taxonomy/Biogeography Hypericum hookerianum Random amplified polymorphic DNA Clonal uniformity Micropropagation
Content Type	Text
Resource Type	Article
Subject	Plant Science

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A high efficiency in vitro regeneration protocol and clonal uniformity analysis in Hypericum hookerianum Wight & Arn., a lesser known plant of ethnomedicinal and economic importance