NDLI: Novel stereoselective carbonyl reductase from Kluyveromyces marxianus for chiral alcohols synthesis

Content Provider	Springer Nature Link
Author	Li, Hai dong Sun, Zhi hao Ni, Ye
Copyright Year	2013
Abstract	A novel nicotinamide adenine dinucleotide phosphate(NADPH)-dependent carbonyl reductase from Kluyveromyces marxianus(KmCR) was identified, which can convert various prochiral ketone esters and ketone substrates to their corresponding chiral alcohols. KmCR was over-expressed in E. coli BL21(DE3), purified to homogeneity, and characterized. The purified enzyme exhibits the highest activity at 40 °C and pH=6.0. Based on the gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis, the monomeric protein was determined to have a molecular weight of approximate 39000. V $_{max}$ and K $_{m}$ of KmCR are 4.28 μmol·min$^{−1}$·mg$^{−1}$ and 0.41 mmol/L for ketone ester substrate ethyl 2-oxo-4-phenylbutyrate(OPBE), 3.09 μmol·min$^{−1}$·mg$^{−1}$ and 1.21 mmol/L for cofactor NADPH, respectively. Cofactor recycle was achieved by co-expression of KmCR and glucose dehydrogenase(GDH) in E. coli. Recombinant E. coli harboring KmCR and GDH showed moderate asymmetric reduction activity towards various α- and β-ketoesters, diaryl ketone substrates. In an aqueous/butyl acetate biphasic system, the whole-cell biocatalyst was used to prepare ethyl (R)-2-hydroxy-4-phenylbutanoate[(R)-HPBE] in an e.e. of 99.5% with a space-time yield of 433.6 g·L$^{−1}$·d$^{−1}$ and a yield of 80.3% at 270 g/L OPBE.
Starting Page	1140
Ending Page	1148
Page Count	9
File Format	PDF
ISSN	10059040
Journal	Chemical Research in Chinese Universities
Volume Number	29
Issue Number	6
e-ISSN	22103171
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-11-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Asymmetric reduction Chemistry/Food Science Analytical Chemistry Chiral alcohol Physical Chemistry Carbonyl reductase Kluyveromyces marxianus co-Expression Inorganic Chemistry Organic Chemistry
Content Type	Text
Resource Type	Article
Subject	Chemistry Education

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