NDLI: Effects of protein on the detection of chlorobenzenes in fish and their elimination by modified QuEChERS method

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Effects of protein on the detection of chlorobenzenes in fish and their elimination by modified QuEChERS method

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Effects of protein on the detection of chlorobenzenes in fish and their elimination by modified QuEChERS method

Content Provider	Springer Nature Link
Author	Li, Gui zhi Zhang, Xing mei Liu, Pei pei Liu, Yong ming Deng, Qiao qiao
Copyright Year	2013
Abstract	Molecular fluorescence spectrometry, resonance Rayleigh scattering and gas chromatography-mass spectroscopy( GC-MS) were used to study the effect of pure fish serum albumin(FSA) as the model protein on the extraction of three chlorobenzenes(CBs: 1,3-DCB, 1,2-DCB and 1,2,4-TCB; DCB=dichlorobenzene; TCB=trichlorobenzene) in fish samples. The results show that there was a strong binding effect between CB and FSA. In an aqueous solution of 90%(volume fraction) acetone, a slow but full protein denaturation might take place, which would cause the unfolding of protein and the releasing of CBs. Based on these results, a QuEChERS(quick, easy, cheap, effective, rugged and safe) method was modified by replacing the traditional acetonitrile with acetone aqueous solution in the present work. This modified QuEChERS method was applied in the determination of CBs in fish samples. The spiked recoveries and the limits of detection were 80.4%–118.3% and 2.4–7.3 ng/g, respectively. This paper proposes a new strategy by slowing down the protein denaturation and releasing bound organic compounds to enhance the extraction efficiency of CBs in fish samples.
Starting Page	1063
Ending Page	1067
Page Count	5
File Format	PDF
ISSN	10059040
Journal	Chemical Research in Chinese Universities
Volume Number	29
Issue Number	6
e-ISSN	22103171
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-11-06
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chlorobenzene Chemistry/Food Science Analytical Chemistry Modified QuEChERS(quick, easy, cheap, effective, rugged and safe) method Physical Chemistry Inorganic Chemistry Gas chromatography-mass spectroscopy Resonance Rayleigh scattering Fluorescence spectrometry Organic Chemistry
Content Type	Text
Resource Type	Article
Subject	Chemistry Education

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