NDLI: Environmental Metagenomics: The Data Assembly and Data Analysis Perspectives

Content Provider	Springer Nature Link
Author	Shukla, Rohit Nandan Maitra, S. S. Kumar, Vinay
Copyright Year	2015
Abstract	Novel gene finding is one of the emerging fields in the environmental research. In the past decades the research was focused mainly on the discovery of microorganisms which were capable of degrading a particular compound. A lot of methods are available in literature about the cultivation and screening of these novel microorganisms. All of these methods are efficient for screening of microbes which can be cultivated in the laboratory. Microorganisms which live in extreme conditions like hot springs, frozen glaciers, acid mine drainage, etc. cannot be cultivated in the laboratory, this is because of incomplete knowledge about their growth requirements like temperature, nutrients and their mutual dependence on each other. The microbes that can be cultivated correspond only to less than 1 % of the total microbes which are present in the earth. Rest of the 99 % of uncultivated majority remains inaccessible. Metagenomics transcends the culture requirements of microbes. In metagenomics DNA is directly extracted from the environmental samples such as soil, seawater, acid mine drainage etc., followed by construction and screening of metagenomic library. With the ongoing research, a huge amount of metagenomic data is accumulating. Understanding this data is an essential step to extract novel genes of industrial importance. Various bioinformatics tools have been designed to analyze and annotate the data produced from the metagenome. The Bio-informatic requirements of metagenomics data analysis are different in theory and practice. This paper reviews the tools that are available for metagenomic data analysis and the capability such tools—what they can do and their web availability.
Starting Page	71
Ending Page	83
Page Count	13
File Format	PDF
ISSN	22502149
Journal	Journal of The Institution of Engineers (India): Series A
Volume Number	96
Issue Number	1
e-ISSN	22502157
Language	English
Publisher	Springer India
Publisher Date	2015-01-10
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Enzymes Metagenomics DNA Civil Engineering Bioinformatics Environmental engineering
Content Type	Text
Resource Type	Article
Subject	Building and Construction Architecture Mechanical Engineering Agricultural and Biological Sciences Civil and Structural Engineering

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